Purification of total alkaloids berberine.doc

 PAGE \* MERGEFORMAT 13 Purification of total alkaloids berberine Author: Xu Pei-Hu, Gao Yuan, Zhang Xueqiong, Zhang, license [Abstract] Objective Optimization of Coptis the best purification process. Method of purification of total alkaloids that affect the factors that effect a systematic study. The best results of total alkaloid berberine was 10-fold purification of the amount of 0.5% dilute sulfuric acid, with milk of lime adjusted pH 9, put it aside for 2h, filtered, the filtrate adjusted with hydrochloric acid pH1, plus sodium chloride to make salt reached 10% , put it aside for 24h, filtration, sedimentation, washed to pH 5. Conclusion purification of total alkaloids berberine preferred process is stable and feasible. [Keywords:] purification of total alkaloids berberine berberine Abstract: ObjectiveTo optimize the best purification process of total alkaloids from Rhizoma Coptidis.MethodsThe factors that affect the result of purification to total alkaloids were determined.ResultsThe optimum isolation process was as follows, adding 10 time of 0.5% sulphuric acid into Rhizoma Coptidis extract, adjusting to pH 9 by lime cream, standing for 2 hours, filtering, adjusting to pH 1 by hydrochloric acid, adjusting the salinity of solution up to 10% by sodium chloride, standing for 2 hours, washing the precipitation by distilled water to pH 5 . ConclusionThe optimum purification technology of total alkaloids from Rhizoma Coptidis is simple, stable and feasible. Keywords:Rhizoma Coptidis; Berberine hydrochloride; Total alkaloids; Purification Plants for the Ranunculaceae Coptis Coptis Coptis chinensis Franch. Triangular leaves Coptis Coptis deltoidea CY Cheng et Hsiao or a cloud with Coptis teeta Wall.’s Dry roots. Coptis mainly contains the original berberine-type alkaloids, including berberine content in the highest, up to 10%. There is heat dampness, Xiehuo the effectiveness of detoxification of ulcerative colitis is also effective [1,2]. Acid precipitat