Qingdu film positive film on the leukemia cells to support u PA Activity of.doc

 PAGE \* MERGEFORMAT 9 Qingdu film positive film on the leukemia cells to support u PA Activity of Abstract [Objective] To observe the effects of disinfection tablets, keep positive film on leukemic cells u  PA activity. [Methods] SD rats were 24, were randomly divided into 4 groups: control group with normal saline, Ching-ching drug used drug-chip group of films, to support feature-feature-fed group were raised, chemotherapy group received intraperitoneal injection of Ara-C to observe the drug-containing serum on HL  60 u  PA activity. [Results] and blank control group compared with Ara-C and clearing drug films, feature-drug serum raised against HL  60 cell culture supernatant u  PA activity have a certain degree of influence, but in order to Ara - glycosides containing serum reduction u  PA activity effective. [Conclusion] Qingdu film, positive film to support the clinical therapeutic effect may be related to the decrease in patients with leukemia cells in vivo expression of u  PA activity related to the role. Keywords: disinfection tablets; keep positive film; leukemia; HL  60; u  PA Abstract: [Objective] We observed the effect of Qingdupian and Yangzhengpian on the u  PA expression in leukemia cells. [Method] 248 SD rats weigh (210 ± 20) g, half male and half female, divided into 4 groups randomly: blank control (0) group, Qingdupian (Q) group, Yangzhengpian (Y) group and Chemotherapy (H) group.Blank control group were fed physiological saline, Q group were fed Qingdupian juice, Y group fed with Yangzhengpian juice, twice a day, 3.5 days.H group were injected Ara  c through peritoneum, once a day, 4 times in all.Draw blood of rats 1 ~ 2 hours after the last feed, extract the serum, and store the serum at 4 ℃. Treat the cells with RPM1640 containing 10% every kind of serum for 48 hours, precipitate the cells; the supernatant fluid was collected respectively for ELISA essay to test the activity of u  PA. [Result] The laborato