Qingkailing different formulations of baicalin in the Improvement of Determination.doc

 PAGE \* MERGEFORMAT 10 Qingkailing different formulations of baicalin in the Improvement of Determination Of: Zhaolan Fen, Fu Hai Dian, Feng into the instrument [Abstract] Objective improvement in different dosage forms Qingkailing determination of baicalin. Methods using high performance liquid chromatography column was Agilent TC C18 column (150 mm 4.6 mm, 5 m), mobile phase of methanol and water ice acetic acid (volume ratio 55:45:1, detection wavelength was 274 nm, flow rate 1 mL min-1. the results of the concentration of baicalin in 0.01057 ~ 0.2114 mg mL-1 range of linear relationship between peak area and good r = 0.999 9, precision, reproducibility, stability and recovery rate results can meet the analysis requirements. Conclusion The method is feasible and accurate formulations for different drugs Qingkailing provide the basis for revised standards. [Keywords:] Qing Kai Ling, baicalin, HPLC, determination Abstract: Objective To improve the determination method for the content of the baicalin in different preparations of Qingkailing. Methods Baicalin was analyzed on an Agilent TC C18 colum (150 mm 4.6 mm, 5 m). The mobile phase consisted of water methanol acetic acid (55:45:1). The flow rate was 1.0 ml min-1 and the detection wavelength was at 274 nm. Results Baicalin had a good linearity in the range of 0.01057-0.2114 mg mL-1 (r = 0.999 9) with satisfied precision, repeatability, stability and recovery. Conclusion This method was practicable and accurate, and it provided the basis for revision of the standards. Keywords:: baicalin; HPLC; content determination Qingkailing prescriptions from traditional Chinese medicine prescription treatment of acute fever, “Sambo febrile disease” in the Angongniuhuang, by the acid, pigs deoxycholic acid, baicalin, mother of pearl, gardenia, buffalo horn, Radix, gold Silver Flower composition, so far a series of products [1]. its clearing and detoxifying, sedative effect, when the drug is