Qushi Huayu Recipe on non-alcoholic steatohepatitis in rats Cathepsin B and tumor necrosis factor-α expression in.doc

 PAGE \* MERGEFORMAT 2 Qushi Huayu Recipe on non-alcoholic steatohepatitis in rats Cathepsin B and tumor necrosis factor-α expression in [Abstract] The prevention and control rats Qushi Huayu Decoction of non-alcoholic fatty liver in rats. Methods: In addition to the normal control group, five things, Wistar male rats were 30 to use carbon tetrachloride (carbon tetrachloride, CCl4) subcutaneous injection of a joint high-fat low-protein diet to establish non-alcoholic fatty liver model. Self-made model after 2 weeks were randomly divided into model group, Qushi Huayu Decoction group and Kam Lok (dichloro compound Diisopropylamine) group, n = 10, respectively, side and I Qushi Huayu Kanra 10 ml / kg gavage. After 2 weeks subjects, serum alanine aminotransferase (alanine aminotransferase, ALT) activity and tumor necrosis factor-α (tumor necrosis factor  α , TNF  α ) content, liver triglyceride (triglyceride, TG), and free fatty acids (free fatty acid, FFA) content; and analyze the correlation among the indicators. Western blotting detected liver cathepsin B (cathepsin B, Ctsb), phospho-κ B inhibitory protein (phospho  inhibitor kappa B, P  Iκ B), and TNF  α protein expression. Immunohistochemical staining of liver tissue Ctsb expression. Results: Qushi Huayu may significantly reduce the model of rat liver tissue TG (P