Rabbit VX2 tumors in vitro and the biological characteristics of.doc

 PAGE \* MERGEFORMAT 17 Rabbit VX2 tumors in vitro and the biological characteristics of Authors: Chang Su, ZHANG Hui-Zhong, Wen-Hai Li, Lin Fang, Liang Xiao-hua, JIANG Lu, Cheng QS [Keywords:] tumor cells Culture in vitro and related biological characteristics of rabbit VX2 carcinoma cells [Abstract] AIM: To separate and purify the rabbit VX2 carcinoma cell line in vitro and to characterize its biological features. METHODS: VX2 tumor fragments were isolated and cultured with explant culture techniques and enzymatic digestion in vitro. After 40 passages, the cell morphology , cell cycle, karyotype and tumorigenecity in rabbits and nude mice were investigated. RESULTS: The newly established cell line VX2 was maintained in continuous culture over 50 passages for 8 months. Morphologically, VX2 cells were uniform, round and polygonal epithelial cells. The cells were small but had a high nucleocytoplasmic ratio. The cell cycle analysis showed 74.61% in G1 phase, 7.78% in G2 phase and 17.6% in S phase. The population doubling time was 26.4 h. The chromosomal analysis showed a hypotriploid with a median chromosome number of 60. The tumorigenecity in rabbits and nude mice will be ensured under a high VX2 cell inoculating concentration. CONCLUSION: The VX2 is a squamouscell carcinoma resulting from malignant change in a papilloma induced with the Shope virus in rabbits. It has already been purified in vitro, and can be applied to further researches. [Keywords] rabbits; tumor cells, cultured; tumor markers, biology [Abstract] Objective: In vitro Isolation and Purification of VX2 tumor cells, to observe its biological properties. Methods: The tissue digestion method and the combination of rabbit VX2 tumors in primary culture, physical rumor on behalf of the observations, passaged 40 times and cultured cells morphology, cell cycle checkpoint, karyotype analysis, rabbits and nude mice. Results: The purified form of the same rabbit VX2 tumor cells, showing round, polyg