Qingfei Oral Liquid on 3I 7b adenovirus infection in human embryonic lung fibroblasts TGF-β1 PDGF-BB mRNA gene expression_0.doc

 PAGE \* MERGEFORMAT 12 Qingfei Oral Liquid on 3I 7b adenovirus infection in human embryonic lung fibroblasts TGF-β1 PDGF-BB mRNA gene expression Of: WANG Wen-ge, CHEN Si Wen, Wang Shouchuan [Abstract] Objective To observe the oral solution containing serum on Qingfei adenovirus 3I, 7b infected human embryonic lung fibroblast transforming growth factor-β1 (TGF-β1), platelet-derived growth factor-BB (PDGF-BB) mRNA gene expression. Methods 3I, 7b, respectively, against adenovirus in vitro cultured human embryonic lung fibroblasts, oral liquid preparation containing serum Qingfei, role in the cell, a separate group of normal cells, the virus control group, ribavirin ribavirin group, detected by in situ hybridization TGF-β1, PDGF-BB mRNA expression, were compared among groups. Results in control group than in normal cells, the virus group, TGF-β1, PDGF-BB mRNA expression were significantly increased (P lt;0.01), containing serum group 3I, 7b-type attack of the TGF-β1, PDGF-BB mRNA expression were significantly lower effect (P lt;0.01). Conclusion Qingfei oral liquid can be reduced TGF-β1, PDGF -BB mRNA expression, which may be one of the mechanisms of its antiviral activity. [Keywords:] Qingfei oral, human embryonic lung fibroblast cells, adenovirus, transforming growth factor-β1, platelet-derived growth factor-BB mRNA, containing serum Abstract: Objective To study the effect of Qingfei oral liquid contained serum on the transforming growth factor-β1 (TGF-β1) and platelet-derived growth factor-BB (PDGF-BB) mRNA gene expression of human embryonic lung fibroblast cells infected by adenovirus (ADV) type 3I and 7b. Method TGF-β1 and PDGF-BB mRNA expression of human embryonic lung fibroblast cells infected by ADV type 3I and 7b were determined by in situ hybridization before and after treated with Qingfei oral liquid contained serum. Results ADV could up-regulate TGF-β1 and PDGF-BB mRNA of human embryonic lung fibroblast cells (P lt;0.01), while Qingfei oral