Radix Paeoniae Rubra and Radix Paeoniae Alba by capillary electrophoresis fingerprint of herbal medicine methodological study.doc

 PAGE \* MERGEFORMAT 24 Radix Paeoniae Rubra and Radix Paeoniae Alba by capillary electrophoresis fingerprint of herbal medicine methodological study Author:王磊磊 , CHEN Jun-hui, WANG Hong, Yin-Fen, WANG Xiao-Ru, YANG Dongfang [Abstract] Objective To determine the peony Paeonia and high-performance capillary electrophoresis methods, the establishment of red peony and white peony root by high performance capillary electrophoresis (HPCE) fingerprints. HPCE method of working conditions: use of uncoated fused silica capillary (inner diameter of 75 μ m, effective length 50 cm), separation voltage 25 kV, column temperature 25 ℃ , diode array detector (DAD) detection wavelength was 220 nm, buffer solution 30 mmol / L borax (pH = 9.0) solution. Click here to conditions on the origin of the seven kinds of different samples and eight kinds of peony Paeonia samples were analyzed. Resulted in the creation and peony Paeonia HPCE fingerprints, using traditional Chinese medicine fingerprints similarity calculation software to control the system-generated fingerprints of different samples as a control template of the map for similarity calculations. Conclusion The method is simple, effective, and can be used for medicinal peony Paeonia and quality control. [Keywords:] Paeonia; TGP; high performance capillary electrophoresis; fingerprint Abstract: ObjectiveTo set up a modern High Performance Capillary Electrophoresis analysis method for separating and detecting Radix paeoniae Alba and Radix paeoniae Rubra and establish their fingerprints. MethodsSeven samples of Radix paeoniae Alba and eight samples of Radix paeoniae Rubra were carried out by HPCE under the following conditions: bared fused silica capillary (50 cm * 75 μ m id), 30 mmol / L borate (pH = 9.0) as buffer, the run voltage is 25 kV, detection wavelength of UV at 220 nm, and column temperature of 25 ℃ . ResultsThe fingerprints were confirmed and compared by the software of the similarity evaluation system for chrom