Receptor-mediated cmyc antisense oligonucleotide inhibited the proliferation of hepatocellular carcinoma Bel7402 cells the mechanism.doc

 PAGE \* MERGEFORMAT 2 Receptor-mediated cmyc antisense oligonucleotide inhibited the proliferation of hepatocellular carcinoma Bel7402 cells the mechanism [Abstract] Objective To investigate the galactose receptor-mediated c  myc antisense oligonucleotide (antisense oligodeoxynucleotide, ASODN) inhibit liver cancer Bel  7402 cell proliferation mechanisms. Method of c  myc ASODN and galactose (galactose, Gal)  PEI (polyethyleneimine, PEI) phase role in the formation of Gal  PEI  ASODN complex, acting on the human hepatoma Bel  7402 cells detected by flow cytometry the cell cycle, AnnexinV  FITC and PI (Propidium Iodide, PI) double staining, DNA gel electrophoresis experimental observation Gal  PEI  ASODN pairs of Bel  7402 cells. The results using flow cytometry cell cycle, cell group, Gal  PEI group, ASODN control group, cell proliferation indices were 35.04%, 33.95%, 32.90%, Gal  PEI  ASODN cell proliferation index of 23.65% with the cells compared to controls, sub  G1 phase + G0/G1 phase cells from a total of 64.03% to 76.74%, S phase + G2 / M phase cells reduced from 35.04% to 23.65%, Gal  PEI  ASODN cell proliferation index fell 11.39%, significantly different (P