Recombinant CD200 gene in COS-7 cells in transient expression.doc

 PAGE \* MERGEFORMAT 11 Recombinant CD200 gene in COS-7 cells in transient expression [Abstract] Objective To study built on the recombinant eukaryotic expression vector pcDNA3-CD200 on the CD200 gene in COS-7 cells in transient expression. Method of in vitro amplification and restriction enzyme digestion pcDNA3-CD200 plasmid; conventional methods cultured COS-7 cells; electricity transfected genes law will pcDNA3-CD200 plasmids were transformed into COS-7 cells, using flow cytometry (FCM) detection of transfected cells, CD200 expression. Results transfected COS-7 cells after 72h, CD200 gene expression positive cell count rate was 44.5%. Conclusion Successful use of electricity transfected gene approach will be recombinant eukaryotic expression vector pcDNA3-CD200 was transfected into COS-7 cells, and implemented in the COS-7 cells transiently expressed, in order to further study the biological activity of CD200, as well as signal transduction laid the foundation. [Keywords:] CD200; COS-7 cells; electricity Transfection; flow cytometry [Abstract] Objective The transient expression of CD200 gene which was constructed to the recombinant eukaryotic expression vector pcDNA3-CD200 was tested. Methods pcDNA3-CD200 plasmid was amplified and tested by an enzyme cutting technique. COS-7 cell was cultured in vitro and transferred with pcDNA3-CD200 by electrotransfer media method. The expression of CD200 was detected by flow cytometry. Results After the COS-7 cell was transferred for 72 hours, the expressing rate was recorded for 44.5%. Conclusion The recombinant eukaryotic expression vector pcDNA3-CD200 was transferred into COS-7 cells successfully by electrotransfer media method. After being transferred to COS-7 cells, the CD200 protein was expressed, which lays a foundation for further studying biological activity and signal transduction of CD200. [Keywords:] CD200; COS-7 cells; electro transfer; flow cytometry CD200 is a membrane glycoprotein Ⅰ for a new