Recombinant anti-HER2 ScFv - tbid gene expression and its implications for breast cancer SKBr.docVIP

Recombinant anti-HER2 ScFv - tbid gene expression and its implications for breast cancer SKBr.doc

  1. 1、本文档共12页,可阅读全部内容。
  2. 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
  5. 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
  6. 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们
  7. 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
  8. 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
Recombinant anti-HER2 ScFv - tbid gene expression and its implications for breast cancer SKBr

 PAGE \* MERGEFORMAT 12 Recombinant anti-HER2 ScFv / tbid gene expression and its implications for breast cancer SKBr Author: Wang Fang, QIU Xiu-Chun, Wang Lifeng, Xu-ming, ZHAO Jing, Meng Yanling, Jialin Tao, Cheng-Ji, FAN Qing-Yu, YANG Gang [Keywords:] Breast Cancer [Abstract] AIM: To observe the expression and the effect of recombinant antiHER2 ScFv / tBid gene in the transfected SKBr3 cell line. METHODS: Recombinant gene of PE transmembrane domain and tbid was obtained from pcDNAbid plasmid by restriction enzyme digestion, and then cloned into vector pCMV and the recombinant plasmid pCMVrpb was transfected into SKBr3 cells. Expression of recombinant plasmid and morphologic changes of SKBr3 cells were detected by immunofluorescent staining. MTT test was used to show how the cells living status was affected by the gene transfection. RESULTS: The recombinant plasmid was constructed and transfected into SKBr3 cells successfully. The fusion protein was found in the cytoplasm of transfected SKBr3 cells. MTT test and indirect immunofluorescent staining showed the apoptosis of transfected SKBr3 cells. CONCLUSION: Recombinant proapoptotic protein of tBid can be expressed in transfected SKBr3 cells and the expressed fusion protein can induce apoptosis. [Keywords] Bid; breast neoplasms; apoptosis; ScFv antibody [Abstract] Objective: To observe the recombinant anti-HER2 ScFv / tbid gene in SKBr3 cells and its transfected SKBr3 cells. Methods: The pairs of restriction enzyme digestion has been built pcDNAbid plasmid obtained with a PE transmembrane domain and tbid of the recombinant gene will be obtained gene was inserted into the eukaryotic expression vector pCMV single-chain antibody gene downstream of ScFv23e transfected SKBr3 cells. indirect immunofluorescence assay the expression of target protein, MTT assay target gene transfected cell proliferation. Results: The transfected SKBr3 cells, detected the expression of target protein. MTT was found that cell proli

您可能关注的文档

文档评论(0)

hhuiws1482 + 关注
实名认证
文档贡献者

该用户很懒,什么也没介绍

版权声明书
用户编号:5024214302000003

1亿VIP精品文档

相关文档