Recombinant BMP4 - 7 fusion gene adeno-associated virus and its bone marrow stem cells on the biological behavior of.docVIP

 PAGE \* MERGEFORMAT 22 Recombinant BMP4 / 7 fusion gene adeno-associated virus and its bone marrow stem cells on the biological behavior of Of: Yuanshao Hui, yet the sword, Shao Guojun, Liu Bo Chao Gang Society [Abstract] [Objective] recombinant human bone morphogenetic protein 4 / 7 fusion gene adeno-associated virus vector (AAV BMP4 / 7 transfected bone marrow stromal cells (BMSCs), observe its effect on the biological behavior of BMSCs. [Methods] (1 step by RT PCR and gene recombination, was cloned from the placenta BMP4 and BMP7 mature peptide cDNA, for BMP4 / 7 fusion gene was cloned into plasmid pGEM plasmid, (2 from pGEM BMP4 / 7 plasmid cut BMP4 / 7 fusion cloned into the shuttle plasmid recombination in E. coli, constructed in 293 cells, AAV BMP4 / 7 recombinant adeno-associated virus vector, (3 different MOI values #8203;#8203;of AAV BMP4 / 7 transfection of rabbit BMSCs, morphological changes were observed, MTT method curve of cell biology tracings were observed on cell proliferation, to AAV EDFP as control, (4AAV BMP4 / 7 cells transfected with rabbit BMSCs 7,14 d, the line determination of ALP and OC, osteogenic activity observed to AAV EDFP do control. [Results] (1 successfully constructed to carry high titers of BMP4 / 7 fusion gene recombinant adeno-associated virus AAV BMP4 / 7, (2AAV BMP4 / 7 cells transfected BMSCs, cell proliferation activity of good transfection efficiency was 72 %, the cells showed the typical bone changes, 1 × 105 vg / cell in the MOI value of less influence on cell morphology, and transfection efficiency of better than 5 × 104vg/cell (59.38%). (3AAV BMP4 / 7 transfection cells, ALP, OC levels were significantly higher, and with the induced culture after transfection time was positively correlated with statistically significant difference between the control group (tALP = 896.88 P lt;0.001 tOC = 543.24 P lt;0.01). [Conclusion] AAV BMP4 / 7 fusion gene transfection efficiency of cells was induced BMSCs osteogenic ac