Regulating protein expression.docVIP

 PAGE \* MERGEFORMAT 6 Regulating protein expression Author: Pan Xi-ming PAN King Yip ZHANG Jin-Bo Chen Jie [Abstract] Objective To study the hemorrhagic shock in rats TM, TM mRNA changes in the mechanism and parameters attached to the intervention of research. Methods SD rats were divided into: sham shock group, shock group, Ringer’s solution resuscitation group, the Senate attached to the recovery group. 2h after the shock and resuscitation, rats were killed out of liver, Western blotting Measuring changes in TM using RT-PCR method measured TM mRNA changes. Results sham shock group, TM, TM mRNA expression. Shock groups, Ringer’s solution resuscitation group decreased TM expression, TM mRNA expression increases with the sham shock group were significantly statistically significant. Shenfu resuscitation group TM upregulation, TM mRNA expression was significantly lower recovery, compared with the sham shock group was no statistical significance, with the shock group, Ringer’s solution resuscitation group were significantly statistically significant. Conclusion hemorrhagic shock decreased TM protein expression, TM mRNA upregulation, and shock damage. Ringer’s solution on protein C activation system to express no obvious regulatory role. Shenfu through the protective effect of endothelial cells, with anti-shock effect. [Keywords:] hemorrhagic shock thrombomodulin Shenfu [Abstract] Objective To investigate the machenism of thrombomodulin (TM) TM mRNA changes in rats with hemorrhagic shock and the effect of Shenfu injection on them. Methods SD rats were assigned to the following four groups: sham shock; HS group; RS group; SF group. After start resuscitate for 2 hours, the liver of rats were taken out and thrombomodulin was performed with Western blotting, The mRNA expression in liver of thrombomodulin was detected by reverse-transcription polymerase chain reaction (RT-PCR). Results The level of TM in liver obviously decreased in HS group, RS group, the mRNA exp