Research progress of liver metabolism in vitro drug.docVIP

 PAGE \* MERGEFORMAT 15 Research progress of liver metabolism in vitro drug [Abstract] Objective: To introduce methods of drug in vitro hepatic metabolism of the latest developments. Methods: According to the literature in recent years, analysis, synthesis, induction. Respectively warm liver microsomes in vitro incubation method, temperature and incubation of liver cells in vitro method, the liver absorbed slurry were incubated in vitro method are introduced. Results: In vitro hepatic metabolism of research methods have developed rapidly. Conclusion: At present, the major hepatic drug metabolism in vitro methods have advantages and disadvantages, but are essential for the development of new drugs to research tools. [Keywords:] liver metabolism; microsomes, liver; liver cells; pharmacokinetics 0 Introduction Liver is the drug metabolism of a major and important organs, is the main site of drug biotransformation is involved in drug metabolism is rich in a huge dependence on cytochrome P450 mixed-function oxidase enzyme system, the majority of drug-phase Ⅰ and Ⅱ -phase metabolic The response of the liver enzyme system involved in drug occurred, and therefore in vitro drug metabolism models are mainly based on the liver, and its unique advantages and characteristics of the study of drug metabolism is widely used. in vitro drug liver metabolism studies have developed a long time, as compared with the in vivo metabolic studies, in vitro metabolism studies have many advantages, ① metabolism in vitro studies can exclude the interference of a number of factors in vivo, direct observation of the metabolic enzyme substrate selectivity of metabolism, in vivo metabolic studies provide important clues and basis. ② for the metabolic conversion rate is low and the lack of sensitivity of drug detection methods, the in vitro metabolism after all, a very good research tools. ③ metabolism in vitro study of the characteristics of a fast and easy for a large number of comp