Resveratrol in combination with 5.docVIP

 PAGE \* MERGEFORMAT 9 Resveratrol in combination with 5 Author: Li Yan, Xing Ke-fei, LI Fu-Yang, Wang Yun, Yao Libo, Liu Xin-ping Keywords: resveratrol Apoptosispromoting effect of resveratrol combined with 5FU on human breast cancer cell line MCF7 【Abstract】 AIM: To investigate whether 5FU in combination with resveratrol can accelerate the apoptosis of human breast cancer cell line MCF7 in vitro. METHODS: Four breast cancer cell lines (MCF7, MDAMB231, SKBR3 and Bcap37) were incubated with different concentrations of resveratrol or / and 5FU for 48 h, then cell survival rate was measured by MTT assay and cell morphological change was observed by phase contrast microscope. Apoptosis was detected by flow cytometry (Annexin V / PI staining) analysis and Hoechst33258 staining. RESULTS: Resveratrol could inhibit the growth of MCF7, MDAMB231, SKBR3 and Bcap37 to different extents. The IC50 values of resveratrol in MCF7, MDAMB231, SKBR3 and Bcap37 cells were 65, 207, 139 and 213 μmol / L, respectively. The IC50 value of 5FU in MCF7 cells was 13 μmol / L. When 5FU and 65 μmol / L resveratrol were combined, the IC50 value of 5FU in MCF7 cells was decreased to 9 μmol / L. Compared with the treatment with 13 μmol / L 5FU or 65 μmol / L resveratrol alone, the percentage of Annexin V () / PI (-) MCF7 cells increased when these 2 drugs were used in combination. The number of apoptotic MCF7 cells calculated with flow cytometry was accordant with the result got with Hoechst33258 staining. CONCLUSION: Resveratrol interacts synergistically with 5FU to induce the apoptosis of MCF7 cells, which suggests that resveratrol is a secondary chemotherapeutic medicine for treating breast cancer. 【Keywords】 resveratrol; chemotherapy; apoptosis; breast neoplasms 【Abstract】 Objective: To study the co-5FU resveratrol to promote human MCF7 breast cancer cell apoptosis. Methods: The four kinds of human breast cancer cell lines (MCF7, MDAMB231, SKBR3, and Bcap3