Rheumatoid arthritis in rats osteoclast differentiation factor and osteoprotegerin gene expression.docVIP

 PAGE \* MERGEFORMAT 14 Rheumatoid arthritis in rats osteoclast differentiation factor and osteoprotegerin gene expression Authors: Han Bing, Zhang Ying, Wu Guang-Heng, Xu Yongzhong [Abstract] Objective In this study, rats with ankle rheumatoid arthritis as experimental models, using RT-PCR detection of rat ankle joint tissues of osteoclast differentiation factor (ODF / RANKL), osteoprotegerin (OPG) in investigate the expression of joint tissue RANKL, OPG expression in bone resorption and rheumatoid arthritis, the molecular mechanisms of damage. Methods The experimental animal models. Reverse transcription - polymerase chain reaction (RT-PCR) was used to determine the experimental group at different time ankle joint tissues of rat RANKL, OPG mRNA gene expression. Results were detected by RT-PCR in rat ankle joint tissues of RANKL and OPG mRNA levels, and to compare its contents, in the normal control group, OPG mRNA levels higher than RANKL mRNA, but as the model was successfully constructed, RANKL / OPG mRNA content ratio in upside down, RANKL mRNA levels gradually increased, the ratio is 4 weeks when 9:1,6 weeks when 6.4:1. Conclusion With the severity of joint destruction, RANKL mRNA expression of tissue increased, OPG expression also increased, RANKL / OPG levels are lower than the ratio of increase of OPG increased significantly in the model building 4 weeks reached 9:1, indicating that RANKL with the expression of OPG ratio and osteoclast function, activity, differentiation and maturity was positively correlated. Local micro-environment in the relative ratio of RANKL and OPG in bone tissue in the decision osteoclasts and osteoblasts function of orientation, the decision is a partial bone absorption, destruction, or proliferation, but also determine the extent of bone destruction. [Keywords:] rheumatoid arthritis, ankle osteoclast differentiation factor osteoprotegerin gene expression in Gene expression of osteoclastogenesis inhibitory factor and osteo