Rhizoma Imperatae Extraction and Determination.docVIP

 PAGE \* MERGEFORMAT 13 Rhizoma Imperatae Extraction and Determination Of: Wang Haixia, Wu Yun, time-dimensional static, Gao best [Abstract] Objective To optimize the extraction of polysaccharides from Rhizoma Imperatae and its content were determined. Methods Rhizoma Imperatae polysaccharide extract was the indexes, the use of orthogonal test the temperature of the extraction process, frequency, time and solid to liquid ratio of four Optimization of factors, the use of phenol - sulfuric acid method for the determination of polysaccharide content. polysaccharide extraction times on the results of the impact of the most significant, followed by the extraction temperature, extraction time and water consumption of their less affected. Conclusion The optimum extraction of polysaccharide Rhizoma Imperatae conditions is 15 times the amount of water reflux extraction temperature 85 ℃ 3 times, each time the water extraction time was 3 h. The polysaccharide content in both origin were more than 0.15%. [Keywords:] Rhizoma Imperatae; polysaccharide; orthogonal test; phenol - sulfuric acid content; Abstract: Objective To optimize the extracting conditions of polysaccharide from Imperata Cylindrica and establish a suitable method for its content determination. Methods Based on the qualitative yield of polysaccharide extraction, a orthogonal test was employed to evaluate the effect of four factors including extracting temperature, extracting time, the extraction times and the ratio of material to liquid. The phenol-dense sulphuric acid method was applied for the content determination of polysaccharide. Results The times of extraction and the extracting temperature influenced the content of polysaccharides mostly, while the duration of extraction and the amount of water showed little influence. Conclusions The optimum extraction condition was refluxing and extracting for 3 times at 85 ℃ for 3 hours, and with 15 folds of water for every time. The contents of polysa