Rhubarb by high performance liquid chromatography worm pills paeoniflorin.doc

 PAGE \* MERGEFORMAT 8 Rhubarb by high performance liquid chromatography worm pills paeoniflorin [Abstract] Objective To establish a high-performance liquid chromatography Rhubarb Pills insects paeoniflorin method. Methods Hypersil ODS2 column (4.6 mm * 250 mm * 5 μ m); mobile phase of acetonitrile -0.1% phosphoric acid (15:85); column temperature 30 ℃ ; detection wavelength of 230 nm. The results of paeoniflorin linear range 0.12 ~ 1.08μ g, the average recovery = 98.78%, RSD = 1.35%. Conclusion The method was simple, reliable, accurate, and can be used for the preparation of quality control in the TGP. [Keywords:] Rhubarb worm pill HPLC paeoniflorin Abstract: ObjectiveTo establish a method for determination of Paeoniflorin in Dahuang Zhechong Pill. MethodsAn HPLC method was developed and the Hypersil ODS2 column (4.6 mm * 250 mm * 5 μ m) was used.The mobile phase was acetonitrile-0.1% orthophosphoric acid (15 : 85). The detection wavelength was at 230nm and the column temperature was 25 ℃ . ResultsThere was a good linear correlationship in the range of 0.12 ~ 1.08μ g, the average recovery was 98.78%, RSD = 1.35%. ConclusionThe method is convenient, reliable and accurate.It is suitable for determination of the content of Paeoniflorin in Dahuang Zhechong Pill. Keywords:: Dahuang Zhechong Pill; Paeoniflorin; HPLC Close Dahuangzhechong pill is contained in the ‘Chinese Pharmacopoeia Ⅰ The 2005 edition of the Department of [1], mainly by the cooked rhubarb, Eupolyphaga worms, leeches, peach seed, peony root, skullcap, etc. composed of 12 Chinese herbal medicines, the effectiveness of promoting blood circulation Huayu, pass through the elimination syndrome. For the bleeding stopped within Erzhi abdominal mass, and the skin a mistake, not do so by the close, the clinical curative effect. But the original drug to Honey pill, dosage form behind them in this experiment to be secondary development, the original Fang Medicine Smell more, ‘the Chinese Pharmacopoeia’