RNAi gene silencing PRL1 invasion of lung cancer cells.docVIP

RNAi gene silencing PRL1 invasion of lung cancer cells.doc

  1. 1、本文档共13页,可阅读全部内容。
  2. 2、原创力文档(book118)网站文档一经付费(服务费),不意味着购买了该文档的版权,仅供个人/单位学习、研究之用,不得用于商业用途,未经授权,严禁复制、发行、汇编、翻译或者网络传播等,侵权必究。
  3. 3、本站所有内容均由合作方或网友上传,本站不对文档的完整性、权威性及其观点立场正确性做任何保证或承诺!文档内容仅供研究参考,付费前请自行鉴别。如您付费,意味着您自己接受本站规则且自行承担风险,本站不退款、不进行额外附加服务;查看《如何避免下载的几个坑》。如果您已付费下载过本站文档,您可以点击 这里二次下载
  4. 4、如文档侵犯商业秘密、侵犯著作权、侵犯人身权等,请点击“版权申诉”(推荐),也可以打举报电话:400-050-0827(电话支持时间:9:00-18:30)。
  5. 5、该文档为VIP文档,如果想要下载,成为VIP会员后,下载免费。
  6. 6、成为VIP后,下载本文档将扣除1次下载权益。下载后,不支持退款、换文档。如有疑问请联系我们
  7. 7、成为VIP后,您将拥有八大权益,权益包括:VIP文档下载权益、阅读免打扰、文档格式转换、高级专利检索、专属身份标志、高级客服、多端互通、版权登记。
  8. 8、VIP文档为合作方或网友上传,每下载1次, 网站将根据用户上传文档的质量评分、类型等,对文档贡献者给予高额补贴、流量扶持。如果你也想贡献VIP文档。上传文档
查看更多
RNAi gene silencing PRL1 invasion of lung cancer cells

 PAGE \* MERGEFORMAT 13 RNAi gene silencing PRL1 invasion of lung cancer cells Of: Zhongxi Ming, Fan Yu, Zhang Ruijia, Zheng Jinxu [Abstract] Objective: To investigate the RNA interference (RNA interference, RNAi) silencing to promote regeneration phosphatase 1 (phosphatase of regenerating liver cell 1, PRL 1) gene on invasion of lung cancer cells. Methods: PRL 1 gene by small interfering RNA (small interfering RNA, siRNA transfection treatment of lung cancer A549 cells, respectively, using real time quantitative RT PCR and Western blot detection of PRL 1 gene mRNA and protein levels, respectively, soft agar culture test and Boyden chamber model test for detection of cancer cells anchor the independent growth and invasion. Results: Compared with control, siRNA transfection group PRL 1 mRNA and protein levels were significantly reduced in a dose and time dependent manner (P lt;0.05, P lt;0.05. vitro study found, PRL 1 siRNA can effectively inhibit cancer cell colony growth and invasion in a dose-related (P lt;0.05, P lt;0.05. Conclusions: PRL 1 gene in lung cancer plays an important role in the invasion, the use of PRL 1 siRNA transfection could inhibit lung cancer cell invasion. [Keywords:] lung cancer, promote liver regeneration phosphatase 1, invasion, RNA interference, small interfering RNA [Abstract] Objective: To study the effects and mechanism of phosphatase of regenerating liver cell 1 (PRL 1small interfering RNA (siRNAon invasion of human lung cancer cell.Methods: After lung cancer cell line A549 were transfected by PRL 1 siRNA, the mRNA and protein of PRL 1 were determined by real time RT PCR and western blot assay, respectively.The anchorage independent growth was exmined by clon formation in soft agar, and invasion ability was evaluated by boyden chamber model. Results: The siRNA could down regulate the level of mRNA and protein of PRL 1 in a dose and time dependent manner. Suppression of of PRL 1 expression can inhibit invasion ability a

您可能关注的文档

文档评论(0)

hhuiws1482 + 关注
实名认证
文档贡献者

该用户很懒,什么也没介绍

版权声明书
用户编号:5024214302000003

1亿VIP精品文档

相关文档