RNAi gene silencing PRL1 invasion of lung cancer cells.docVIP

 PAGE \* MERGEFORMAT 13 RNAi gene silencing PRL1 invasion of lung cancer cells Of: Zhongxi Ming, Fan Yu, Zhang Ruijia, Zheng Jinxu [Abstract] Objective: To investigate the RNA interference (RNA interference, RNAi) silencing to promote regeneration phosphatase 1 (phosphatase of regenerating liver cell 1, PRL 1) gene on invasion of lung cancer cells. Methods: PRL 1 gene by small interfering RNA (small interfering RNA, siRNA transfection treatment of lung cancer A549 cells, respectively, using real time quantitative RT PCR and Western blot detection of PRL 1 gene mRNA and protein levels, respectively, soft agar culture test and Boyden chamber model test for detection of cancer cells anchor the independent growth and invasion. Results: Compared with control, siRNA transfection group PRL 1 mRNA and protein levels were significantly reduced in a dose and time dependent manner (P lt;0.05, P lt;0.05. vitro study found, PRL 1 siRNA can effectively inhibit cancer cell colony growth and invasion in a dose-related (P lt;0.05, P lt;0.05. Conclusions: PRL 1 gene in lung cancer plays an important role in the invasion, the use of PRL 1 siRNA transfection could inhibit lung cancer cell invasion. [Keywords:] lung cancer, promote liver regeneration phosphatase 1, invasion, RNA interference, small interfering RNA [Abstract] Objective: To study the effects and mechanism of phosphatase of regenerating liver cell 1 (PRL 1small interfering RNA (siRNAon invasion of human lung cancer cell.Methods: After lung cancer cell line A549 were transfected by PRL 1 siRNA, the mRNA and protein of PRL 1 were determined by real time RT PCR and western blot assay, respectively.The anchorage independent growth was exmined by clon formation in soft agar, and invasion ability was evaluated by boyden chamber model. Results: The siRNA could down regulate the level of mRNA and protein of PRL 1 in a dose and time dependent manner. Suppression of of PRL 1 expression can inhibit invasion ability a