Roots and stems and leaves in different seasons of Emodin in Polygonum content analysis_0.docVIP

 PAGE \* MERGEFORMAT 10 Roots and stems and leaves in different seasons of Emodin in Polygonum content analysis Of: Huguan Yu, Xia Wake up, Yin G, Chen Qin [Abstract] Objective To determine the medicinal plant Polygonum cuspidatum at different seasons in its different parts of the active ingredient in the growth of emodin and its variation, is designed to Polygonum provide the basis for the best harvest time. Methods HPLC , Diamonsil-C18 (250 mm × 4.6 mm, 5 μm) column, methanol -0.1% phosphoric acid (80:20) as mobile phase, detection wavelength: 254 nm, flow rate: 1.0 mL / min, column temperature: 27 ℃, injection volume: 20 μL. results in different seasons by measuring the stems and roots of Polygonum emodin content, the highest content found in the root of emodin and emodin in the highest in August. Conclusion cuspidatum in the growth and development process, rhubarb element content is regular, according to its root in the emodin content to determine the optimal harvest time. [Keywords:] cuspidatum, emodin, harvest time, high performance liquid chromatography Abstract: Objective To find the changing regularity of emodin content in different tissues of Polygonum cuspidatum gathered in different seasons by HPLC, so to decide the best gathering time. Methods The separation was performed on Diamonsil-C18 (250 mm × 4.6 mm, 5 μm ) column, using methanol-0.1% H3PO4 (80:20) as mobile phase, detected at 254 nm. The flow rate was 1.0 mL / min, the column temperature was 27 ℃. Results Among the rhizome and stem leaf of Polygonum cuspidatum, the content of emodin in the rhizome was the highest. Over a year, the content of emodin was the highest in august. Conclusion The content of emodin changes regularly in the process of growth and development of Polygouum cuspidatum, The best gathering time could be determined by the emodin content in the rhizome of Polygouum cuspidatum. Keywords:: Polygonum cuspidatum, emodin, gathering time, HPLC Polygonum (Polygonu