Rhodiola Rhodiola glucosinolate content in wine by HPLC.doc

 PAGE \* MERGEFORMAT 9 Rhodiola Rhodiola glucosinolate content in wine by HPLC Of: Yang Daobin, Liu Li, Jia Guofu, Liu Sujun [Abstract] Objective Rhodiola rosea by high performance liquid chromatography salidroside content of the wine. Methods column was Shim-pack VP-ODS C18 (250 mm × 4.6 mm, 5.0 μm), mobile phase methanol - water (eighty-two past six p.m.), the detection wavelength was 278 nm, flow rate was 1.0 ml / min, column temperature was 30 ℃. salidroside sample volume results in the range of 0.072 ~ 1.296 μg and the peak area was a good line integral value sexual relationship (r = 0.999 9), the average recovery was 98.90%, RSD 1.32%. Conclusion The method is sensitive, simple, accurate and reliable and can be used Rhodiola wine Determination of salidroside quality control and evaluation. [Keywords:] Rhodiola wine; Salidroside; high performance liquid chromatography Abstract: ObjectiveTo establish an HPLC method for the quantitative determination of salidroside in Hongjingtian wine. MethodsThe samples were separated on a Shim-pack VP-ODS C18 (250 mm × 4.6 mm, 5.0 μm) column with the mobile phase of methanol-water ( 18:82) in the flow rate of 1.0 ml · min-1. The detection wavelength was set at 278 nm. column temperature was 30 ℃. ResultsThe linear range of salidroside was 0.072 ~ 1.296 μg (r = 0.999 9). The average recovery of salidroside was 98.90 and the RSD was 1.32% (n = 5). ConclusionThis method is sensitive, simple and accurate, which can be used to evaluate the quality control of Hongjingtian wine. Keywords:: Hongjingtian wine; Salidroside; HPLC Sedum Rhodiola plants for the Rhodiola (Rhodiola crenulata) dry root and rhizome, or the whole plant medicine, with heat and detoxifying, dampness, nourishing the strong, strengthening and restoring the function [1], was Emperor Kangxi Royal called “Sin gives grass” plateau ginseng. Rhodiola wine (wine Suluo Po Ma) developed by the Grassland Research Institute of Sichuan Province, there ar