Rhododendron leaves improved CTAB method of total RNA extracted.doc

 PAGE \* MERGEFORMAT 13 Rhododendron leaves improved CTAB method of total RNA extracted Of: Guo Xiulian, Zhang Zheng-yin, Tian Ping, Luo Shao silver, Bai Jie, ZHUANG Ping [Abstract] Objective To study the rhododendron leaves the extraction of total RNA. Methods for rich secondary metabolites of a rhododendron leaf total RNA extraction method - modified CTAB method. Using CTAB as detergent, respectively, with chloroform and water saturated phenol extraction and repeated precipitation high concentration of NaCl to remove proteins, polysaccharides and secondary metabolites and other impurities, and finally precipitated with ethanol to obtain total RNA. The results obtained by this method not only makes a good total RNA integrity high purity, and simple, fast and low cost. Conclusions The method of azaleas and other plants rich in secondary metabolites, especially rich in polyphenols, polysaccharides extracted material with reference total RNA. [Keywords:] RNA extraction, azaleas, modified CTAB method ChinaAbstract: ObjectiveTo study the method for isolation of total RNA from Rhododendron leaf. MethodsAn improved CTAB method was presented here to be suitable for isolation of total RNA from Rhododendron leaf, a plant which contained abundant secondary metabolites. Using chemical CTAB as detergent in the extraction buffer, the absolute ethanol precipitation of RNA was performed finally after removing of proteins, polysaccharides, and secondary metabolites by subsequent chloroform, H2O-saturated phenol extraction and high concentration NaCl precipitation, respectively.ResultsThe yielded total RNA had integrality and high purity but. Conclusion The method may be applied to some difficult RNA extraction plants full of polyphenol and polysaccharides, particularly Rhododendron. Keywords:: RNA extraction; Rhododendron; Improved CTAB Method Rhododendron mosaic film of protein, polysaccharides, polyphenols and other secondary substances in many and comple