RP-HPLC determination of Selaginella from different areas in A Mantuo pairs of Flavonoids.doc

 PAGE \* MERGEFORMAT 10 RP-HPLC determination of Selaginella from different areas in A Mantuo pairs of Flavonoids Authors: Xiao-Ke Zheng, Zhao Xian-Min, Wang Yan Zhi, Yue Wei, Guo Yonghui, Feng health [Abstract] Objective To determine the different areas in A Mantuo pairs of Selaginella flavonoids by high performance liquid chromatography. Method column was Agilent ZORBOX SB-C18 (250 mm * 4.6 mm, 5 μ m); mobile phase of acetonitrile -0.2% phosphoric acid (40:60), column temperature 30 ℃ ; a flow rate of 0.8 ml * min-1; detection wavelength 337 nm. Results A Mantuo pairs of flavonoids content of 1.197 percent, the average recovery was 99.30%, RSD = 0.98% (n = 9), the linear range of 0.1 ~ 0.6 μ g * ml-1 (r = 0.999 8). Conclusion The method fast, sensitive, accurate and can be used as reference for the quality of Selaginella medicinal herbs. [Keywords:] Volume Boa Manto pairs of flavonoids by high performance liquid chromatography For Selaginella Selaginella Selaginella Selaginella tamariscina (Beauv.) Sping or cushion-like Selaginella Selaginella pulvinata (Hook. et Grev.) Maxim) dry the whole plant, with blood circulation pass through effect, mainly used for amenorrhea dysmenorrhea, Zhengjia lump in the abdomen or flutter injury, uterine bleeding, hematochezia, anal prolapse, etc. [1] disease. According to the literature reports [2 ~ 4], the main Selaginella A Mantuo pairs containing flavonoids, the new Japanese cedar pairs of flavonoids and other compounds with anti-lipid peroxidation and free radical scavenging effects [5], on the hemolytic phosphatidylcholine (LPC)-induced endothelial cell injury has a protective effect [6], where A Mantuo pairs of Flavonoids in the greatest. RP-HPLC fingerprint of Selaginella medicinal research reports have been seen, but no Selaginella medicinal herbs from different areas in A Mantuo pairs of flavonoids determination of coverage. In this study, high-performance liquid chromatography determination of 10 different grou