RP-HPLC determination of the content of hyperin in Yuzhong.doc

 PAGE \* MERGEFORMAT 9 RP-HPLC determination of the content of hyperin in Yuzhong [Abstract] Objective To establish the manner in Yuzhong hyperin high performance liquid chromatography assay method. Methods RP-HPLC determination, C18 column, mobile phase was methanol -0.2% phosphoric acid (46:54), with triethylamine pH adjusted to 3.0, flow rate 1.0 ml * min-1, detection wavelength 370 nm; for the test product Preparation of solution ultrasonic extraction with methanol. The results measured in different batches from different areas of Burnet medicine, hyperin mass fraction at 0.052% ~ 0.291%. Conclusion The method is sensitive and rapid, accurate and reliable, can be used for quality control of Sanguisorba. [Keywords:] Burnet hyperin RP-HPLC Abstract: ObjectiveTo establish a determination method of hyperoside in Sanguisorba officinalis. MethodsRP-HPLC analytical method was established on a Polaris C18column with Methanol-0.2% H3PO4 solution (46:54) as the mobile phase, the flow rate was 1.0 ml * min - 1, and the detection wavelength was 370 nm. The method of sample was ultrasonic wave withdraws by Methanol. ResultsThe content of hyperoside was from 0.052 ~ 0.291% in different groups of S. officinalis collected from the different locations. ConclusionThe method is accurate and can be used for the quality of Sanguisorba officinalis. Keywords:: Sanguisorba officinalis; Hyperoside; RP-HPLC For the Rosaceae Sanguisorba Sanguisorba Sanguisorba officinalis L. plants or long-leaf Sanguisorba Sanguisorba officinalis L.var.longifolia (Bert.) Yu et Li of dry root. The bitter, sour, astringent, slightly cold, with a cooling blood to stop bleeding sore of the effectiveness of detoxification convergence [1,2]. Modern pharmacological studies have shown that its has significant analgesic effect [3], the main active ingredients flavonoids, but the record quality standards Pharmacopoeia method of quantitative detection of only ultraviolet - visible spectrophotometry of