RP-HPLC determination of the film re-House Jidesheng Yiyao content of saponin I.doc

 PAGE \* MERGEFORMAT 9 RP-HPLC determination of the film re-House Jidesheng Yiyao content of saponin I [Abstract] Objective To establish a RP-HPLC determination of the film re-House Jidesheng Yiyao content of saponin I. Methods Agilent Zorbax SB C18 column (4.6 mm * 250 mm, 5 μ m); mobile phase of acetonitrile - water (45:55); flow rate of 1.0 ml / min, column temperature 30 ℃ , detection wavelength was 210 nm. The results re-floor saponin I in the 0.009 645 ~ 0.964 5 mg / ml within the range of peak area showed good linear relationship, r = 1.000 0; The average recovery was 99.21%, RSD of 0.75% (n = 9). Conclusion The method is simple, accurate, good reproducibility can be used to determine the film re-House Jidesheng Yiyao I content of saponin. [Keywords:] re-floor saponin Ⅰ ; Jidesheng snake tablets; high performance liquid chromatography Abstract: ObjectiveTo develop an HPLC method for determination of Chonglou Saponin I in Jidesheng Sheyao tablets. Methods The HPLC method was used with Agilent Zorbax SB C18 column (4.6 mm * 250 mm, 5 mm), the mobile phase was acetonitrile-water (45 : 55) and the flow rate was 1.0 ml / min. The detection wavelength was 210 nm. ResultsThe method had good linear relationship within the range of 0.009 645 ~ 0.964 5 mg / ml. The average recovery was 99.21% (RSD = 0.75% , n = 9). ConclusionThe method is simple, accurate and reliable. It is appropriate for the determination of Chonglou Saponin I in Jidesheng Sheyao tablets. Keywords:: Chonglou Saponin I; Jidesheng Sheyao tablets; HPLC Jidesheng snake tablet by Aescin a flower, Toad skin, centipedes, Verbena Medicine Smell made of grass, etc., with heat, detoxification, swelling the analgesic efficacy for the treatment of poisonous snakes, poisonous insect bites. The variety of traditional Chinese medicine to protect species, is currently closed is contained in ‘The People’s Republic of China Ministry of Health standards for traditional Chinese medicine set prescription dru