RP-HPLC determination of water extract of propolis phenolic compounds in.doc

 PAGE \* MERGEFORMAT 16 RP-HPLC determination of water extract of propolis phenolic compounds in Of: Chen Fu Yu Bin Phillis Li-yan Points of high Yinyu [Abstract] for the simultaneous determination of aqueous extract of propolis in China, 23 kinds of phenolic compounds in reversed-phase high performance liquid chromatography (RP HPLC analysis. By ZORBAX Eclipse XDB C18 column (150 mm × 4.6 mm × 5 μm, mobile phase : methanol 0.1% formic acid, gradient elution, flow rate 1.0 mL / min, detection wavelength at 256 and 280 nm, injection volume 20 μL, column temperature was 35 ℃. the reference standard chromatographic peak area of #8203;#8203;concentration and a good linear relationship with better accuracy and reproducibility, the standard recoveries between 93.29% ~ 106.61%. use this method, from Hebei detected 18 kinds of propolis reference substance, which was the highest epicatechin 35.50 mg / g WEP , followed by 3,4-dimethyl ammonium acid 15.41 mg / g WEP. propolis of Yunnan reference substance was detected in 9, the highest content of epicatechin 11.23 mg / g WEP, chrysin, followed by 3.61 mg / g WEP, WEP both the HPLC graph similarity propolis were 0.099 (256 nm) and 0.194 (280 nm), significantly different chemical composition. [Key Words] high performance liquid chromatography, propolis and water extract of phenolic compounds 1 Introduction Propolis (Propolis is a bee collecting shoots of plants such as poplar and injured parts of the effusion, processing and transformation in the mouth from a rubbery material. Brittle at low temperatures will harden, become soft when the temperature rises and has a certain viscosity. propolis contains 300 kinds of chemical ingredients, including aromatic and flavonoids, organic acids, terpenoid substances, vitamins, enzymes, etc. [1]. propolis has antioxidant, antibacterial, anti-inflammatory, anti-virus, such as liver protection and anticancer effects [2]. Most of the active ingredients of propolis etha