RP-HPLC determination of wild chrysanthemum Linarin content.docVIP

RP-HPLC determination of wild chrysanthemum Linarin content Author: Wang Botao Wang Feng Zhang Tong-bo WANG Hui-min [Abstract] Objective To establish a wild chrysanthemum Linarin method for the determination. Methods RP-HPLC (RP-HPLC) method, at room temperature, C18 column (4.6 mm * 250 mm, 5 μ m); methanol - water - glacial acetic acid (52:46:2) as mobile phase; The detection wavelength was 334 nm. Results Linarin linear range of 0.05 ~ 2.5μ g, r = 0.999 9 (n = 10); average recovery was 99.2%, RSD = 2.4% (n = 6). Conclusion This method can be used as the quality of medicinal wild chrysanthe