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RP-HPLC fingerprint of Poria Pieces
PAGE \* MERGEFORMAT 9
RP-HPLC fingerprint of Poria Pieces
Of: manganese Lu, He Haihua, Yang Yun, Zhang Hanjuan
[Abstract] Objective To establish the fingerprint of Poria Pieces. Methods HPLC, acetonitrile -0.05% phosphoric acid in water, gradient elution, flow rate: 1.0ml/min, column temperature: 30 , UV detection wavelength: 242 nm . Results Poria Pieces in 11 peaks were identified as the fingerprint peaks, the use of “Chromafinger V0.9 beta” software evaluation fingerprint of 12 batches of Poria Pieces of similarity, correlation coefficients greater than 0.840 3, the angle is greater than the cosine law 0.906 8. Conclusion The method is simple, stable, accurate, reproducible, and can be used for the identification of Poria Pieces, Pieces can effectively control the quality.
[Keywords:] Poria Pieces, high performance liquid chromatography, fingerprinting
Abstract: ObjectiveTo establish HPLC fingerprint of Poria cocos herbs.MethodsHPLC was conducted and the mobile phase consisted of acetonitrile-0.05% phosphoric acid-water was eluted in gradient elution.The flow rate was 1.0 ml / min. The column temperature was at 30 and the detection wavelength was 242nm.ResultsThe eleven common peaks of Poria cocos herbs were determined as fingerprint peaks.Similarity was analyzed by Chromafinger V0.9 beta fingerprint analysis software. The results showed that similarity was more than 84.03% by correlation coefficient method and similarity was more than 90.68% by cosine method. ConclusionThis method is simple, stable and reproducible.It can be used to identify and effectively control quality of Poria cocos herbs.
Keywords:: Poria cocos herbs, HPLC, Fingerprint
Division for the fungus Poria cocos polypore Poria cocos (Schw.) Wolf of dry sclerotia [1], with the benefits of water Shenshi, spleen and soothe the nerves of the effect. Is mainly used for dysuria, edema, diarrhea, spleen, falling more than, phlegm Stop wandering, loss of appetite, palpitation, i
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