RPHPLC Determination of Rehmannia of 5.doc

 PAGE \* MERGEFORMAT 12 RPHPLC Determination of Rehmannia of 5 Author: Xiang-Yang Qin, Zhou Jun, Li Ye, SUN Xiao-Li Keywords: Rehmannia RPHPLC determination of 5hydroxymethylfurfural in prepared rehmannia root 【Abstract】 AIM: To develop a HPLC method for the determination of 5hydroxymethylfurfural (5HMF) in prepared rehmannia root. METHODS: The RPHPLC method was used. The analytical column was a ZODBAXC18 (4.6 mm × 150 mm, 5 μm) and the mobile phase consisted of methanol 0.01 mol / L phosphoric acid aqueous solution (70:30). The wavelength was set at 284 nm with the Detector and the flowrate 0.8 mL / min, the column temperature 25 ℃ and the injection volume 20 μL. RESULTS: Over the range of 0.44-2.18 μg, a good linear relation existed between 5HMF and the peak area (r = 0.999 9, n = 5). The average recovery of the added sample was 99.0% and RSD 0.8% (n = 6) . CONCLUSION: The proposed method can be used for quantitatively analyzing 5HMF in a convenient and economic way, with high accuracy and good reproducibility. This method for the determination of 5HMF in prepared rehmannia root can be popularized. 【Keywords】 radix rehmanniae praeparata; reversed phase highperformance liquid chromatography; 5hydroxymethylfurfural 【Abstract】 Objective: To determine the Rehmannia of 5-HMF (5HMF) content. Methods: The RP-HPLC to ZODBAXC18 (4.6 mm × 150 mm, 5 μm) for column The mobile phase was methanol and 0.01 mol / L phosphoric acid (70:30), detection wavelength of 284 nm, flow rate 0.8 mL / min, column temperature: 25 ℃, injection volume: 20 μL. Results: 5HMF at 0.44 ~ 2.18 μg and the peak area within the linear relationship was good, r = 0.9999,5 HMF average recovery was 99.0%, RSD of 0.8% (n = 6). Conclusion: The use of RP-HPLC method is simple, fast , high reliability, accuracy and good reproducibility, confirming that the detection is worth promote the use of the content of Rehmannia in 5HMF method. Keywords: Rehmannia; RP-HPLC