RTPCR Detection of leukemia stem cells in Flt3 and Nras gene expression in.doc

 PAGE \* MERGEFORMAT 13 RTPCR Detection of leukemia stem cells in Flt3 and Nras gene expression in Author: XU Rui-rong WANG Xiao-ling Wang Yi CUI Xing-Wang Yan [Abstract] Objective: Using reverse transcription  polymerase chain reaction (RT  PCR) to detect Yiqiyangyin parties and their subsequent demolition side righting side group, Quxie party group of acute leukemia stem cells in Flt3 and N  ras gene expression in with a view to clarify the Flt3 and N  ras gene expression and disease occurrence and development of relationships. Methods: RT  PCR Detection of medicine Yiqiyangyin side group, Fuzheng side group, side group and the control group Quxie AML bone marrow mononuclear cells in patients with Flt3 and N  ras gene expression in the case. Results: Flt3 and N  ras gene expression was Yiqiyangyin group, centralizer group, Quxie group and the control group, the difference was significant (P all amp;quot;0.01); Yiqiyangyin group and the centralizer group and Qu evil group, the difference was significant (P all amp;quot;0.01); centralizer group Quxie group difference was not statistically significant (Pamp;gt; 0.05); all FAB subtypes of AML between the expression of Flt3 and N  ras positive rates the difference was not statistically significant (Pamp;gt; 0.05). Conclusion: The traditional Chinese medicine may reduce the Yiqiyangyin Flt3 and N  ras gene expression levels in AML cells, inhibit clonal proliferation of AML cells, have a therapeutic effect on the AML. [Keywords:] leukemia stem cells in RT  PCR Flt3 N  ras The body of the hematopoietic process is complex, affected by a variety of positive and negative regulatory factors of regulation. These hematopoietic growth factors or cytokines in the regulation of imbalance that can lead to excessive proliferation of certain cell lines, and even changes in leukemia. Flt3 receptor (fms  like tyrosine kinase receptor III, FR) is the Lyman discovered in 1993, equivalent to a cell surface