S100A2 in laryngeal squamous cell carcinoma and its significance.docVIP

 PAGE \* MERGEFORMAT 23 S100A2 in laryngeal squamous cell carcinoma and its significance [Abstract] Objective To study the S100A2 protein in laryngeal squamous cell carcinoma (laryngeal squamous cell carcinoma, lscc) protein expression and to explore its in laryngeal squamous cell carcinoma, development, invasion and metastasis. Methods 40 patients Affiliated Hospital of Luzhou Medical College Otolaryngology - Head and Neck Surgery, January 2005-May 2007 surgical pathology confirmed by laryngeal squamous cell carcinoma (LSCC specimens of patients with pathological paraffin as the experimental group over the same period 40 cases of an alternative surgical pathology confirmed no tumor invasion by cancer next to the laryngeal mucosa tissue as a control group. All 4μm paraffin serial sections by two, in which a HE staining, the other one by immunohistochemistry SP method detected the expression of S100A2 protein. the test kit as a positive set of positive biopsy with phosphate buffer (Phosphate Buffer Solution, PBS instead of primary antibody as negative control. EXPERIMENTAL steps with reference to the specific kit instructions, laryngeal squamous cell carcinoma under the microscope, and no tumor infiltration of adjacent cells in the mucosal tissue of the distribution of S100A2 situation and count the positive expression rate. Results laryngeal squamous cell carcinoma and normal laryngeal mucosa S100A2 protein expression in the nucleus and cytoplasm, yellow to brown color .40 patients without tumor invasion of the laryngeal mucosa adjacent all of S100A2 protein positive expression, the expression rate was 100% (40/40), 40 cases of S100A2 protein expression in laryngeal carcinoma was 78% (31/40, expression is lower than the adjacent non-tumor-infiltrating laryngeal mucosa (P lt;0.05, S100A2 protein in different laryngeal squamous cell differentiation positive rate: well-differentiated expression was 91% group, positive expression in the differentiation group was