S1001 marine actinomycetes of anti-tumor activity of constituents of.docVIP

 PAGE \* MERGEFORMAT 15 S1001 marine actinomycetes of anti-tumor activity of constituents of Author: Liu Rui Zhu Weiming Tian-Jiao Zhu Lidong Cui Cheng-Bin, GU Qian-Qun Keywords: marine actinomycetes;, secondary metabolites;, isoflavones;, cyclic peptide;, anti-tumor activity of Abstract: The one derived from the actinomycetes of marine mud, S1001 fermentation product has the activity of cell necrosis induced by this paper, solvent extraction, silica gel column chromatography and the preparation of HPLC separation methods such as fermentation product of the active site to track the separation of active A total of eight compounds were isolated by means of physical and chemical properties and spectroscopy, and their structures were identified as 4 ‘, 5,7 trihydroxy-isoflavone (1), 4 (2,4-Dihydroxy-amide) benzoic acid (2), cyclo-(glycine-alanine) (3), cyclo-(glycine-proline) (4), cyclo-(Leu-tyrosine) (5), cyclo-(Leu-Val acid) (6), Central (valine isoleucine) (7), ring (phenylalanine glycine) (8). SRB method and used a preliminary evaluation of compounds 1 ~ 8 of anti-tumor activity, results showed that the compounds 4,5 and 7 at 10μmol / L concentration of K562 cells showed some inhibitory activity. Keywords: Marine actinomycete; secondary metabolites; isoflavones; cyclic peptide; anti-tumor activity The antitumor active component from marine derived actinomycete S1001 KEY WORDS Marine actinomycete; Secondary metabolites; Isoflavone; Cyclodipeptides; Antitumor activity Marine organisms because of living in high-salt, high pressure and Oligotrophic a special environment, has a unique metabolic approach, it can produce a number of novel structure, activity of a unique secondary metabolites. The past 20 years, the marine micro-organisms to generate new reports of active secondary metabolites gradually increased, marine micro-organisms as a new source of active substances are increasingly attach importance to marine researchers