RP-HPLC determination of Bozhi mycelia three kinds of nucleoside components in.docVIP

 PAGE \* MERGEFORMAT 10 RP-HPLC determination of Bozhi mycelia three kinds of nucleoside components in [Abstract] Objective To establish a high-performance liquid chromatography (HPLC) gradient elution method for simultaneous determination of Bozhi mycelia of three kinds of nucleotide composition (uracil, uridine, adenosine) were measured. Methods RP-HPLC determination, Alltima C18 column (5 μ m, 4.6 mm * 250 mm) as analytical column, mobile phase of acetonitrile - water, gradient elution (0 ~ 15 min, acetonitrile - water ratio of 5:995, 15 ~ 30 min, the ratio of 15:985), flow rate 1.0 ml * min-1, detection wavelength 262 nm. The results of the linear range of three nucleotides, respectively, uracil: 0.140 2 ~ 0.700 8 μ g, (r = 0.999 8, n = 5); uridine: 0.103 2 ~ 0.515 6μ g (r = 0.999 7, n = 5 ); adenosine: 0.124 5 ~ 0.622 4 μ g (r = 0.999 4, n = 5). The average recoveries were: 99.44% uracil, uridine 100.08%, 99.96% adenosine. Conclusion The method is sensitive, easy to operate, good repeatability, high efficiency, for a comprehensive evaluation of the quality of Bozhi mycelia provide a reliable method. [Keywords:] RP-HPLC; Bozhi mycelium; gradient elution; nucleoside composition Abstract: ObjectiveTo determine the contents of nucleosides in Bo Zhi Mycelium (uracil, uridine, adenosine) by HPLC with gradient elution.MethodsUracil, uridine, adenosine were dealt with by HPLC with Alltima-C18 analytical column (5μ m, 250 mm * 4.6 mm) . The mobile plase consisted of acetonitrile-water, gradient elution (0 ~ 15 min, CH3CN-water 5:995,15 ~ 30 min, CH3CN-water 15:985). The flow rate was 1.0 ml * min-1.The UV detection was set at 260 nm.ResultsThe linear ranges of uracil, uridine and adenosine were 0.140 2 ~ 0.700 8 μ g (r = 0.999 8, n = 5); 0.103 2 ~ 0.515 6 μ g (r = 0.999 7, n = 5 ); 0.124 5 ~ 0.622 4μ g (r = 0.999 4, n = 5) respectively.The average recovery was 99.44%; 100.08% and 99.96% respectively.ConclusionThis method is simple, reliable, efficient, and pro