RP-HPLC determination of felodipine extended release tablets.docVIP

 PAGE \* MERGEFORMAT 7 RP-HPLC determination of felodipine extended release tablets [Abstract] Objective: To establish a RP-HPLC determination of felodipine extended release tablets approach. Methods: sinochrom-ODS-BP column (200 mm * 4.6 mm, 5 μ m), with methanol: water (70:30) as mobile phase, UV detection wavelength of 240 nm, flow rate of 1.2 mL / min, column temperature was 30 ℃ . Results: This method in the felodipine concentration 5 μ g / mL ~ 50 μ g / mL range a good linear relationship, the correlation coefficient γ = 0.9996,, day precision RSD ≤ 1.7%. The mean recovery was 99.5%. Conclusion: This treatment is simple, rapid determination of accurate, reproducible, suitable for extended release felodipine tablets in the determination of quality control. [Keywords:] felodipine; RP-HPLC; assay Abstract Objective: A reversed phase HPLC method was developed to determine the contents of felodipine in felodipine sustained-release tablets. Methods: sinochrom ODS-BP column (200 mm * 4.6 mm, 5 μ m) was used, and the mobile phase was composed of methanol-water (70:30). The detection wavelength was at 240 nm with the flow rate at 1.2 mL / min, column temperature was controlled about 30 ℃ . Results: The linear range was 5 μ g / mL ~ 50 μ g / mL for felodipine (γ = 0.9996), The average recoveries of felodipine were 99.5% (RSD ≤ 1.7%). Conclusion: This method appeared to be rapid, simple and accurate. Keywords: Felodipine; RP-HPLC; Content Determination Felodipine is a new dihydropyridine calcium ion channel blockers, mainly for treatment of essential hypertension. Its sustained-release tablet-type with a long-lasting, the advantages of fewer adverse reactions, clinical application of a wide range [1]. Determination of felodipine methods of oxidation-reduction method, UV spectrophotometry [2] and so on, but the poor specificity of these methods affect the accuracy of measurement. Some scholars using high performance liquid chromatography felodipine tablets a