RP-HPLC determination of Danshen capsules in Fukang paeoniflorin.docVIP

 PAGE \* MERGEFORMAT 9 RP-HPLC determination of Danshen capsules in Fukang paeoniflorin [Abstract] Objective To determine women’s Capsule Salvia paeoniflorin method. Method column Reliasil C18 (5 μ m, 250 mm * 4.6 mm), guard column (5μ m, 12.50 mm * 4.6 mm), acetonitrile -0.02 mol * L-1 sodium dihydrogen phosphate solution (26:74) as mobile phase The detection wavelength was 230 nm, column temperature 30 ℃ , a flow rate of 1.0 ml * min-1. The results of paeoniflorin in the 0.496 ~ 29.76 μ g * ml-1 linear range and chromatographic peak integral value of peak area showed a good linear relationship, r = 0.999 5; paeoniflorin average recovery was 98.82%, RSD = 2.11%. Conclusion The method is simple, separation complete, and reliable, able to effectively control the quality of preparation. [Keywords:] paeoniflorin Salvia Women’s capsule by RP-HPLC Abstract: ObjectiveTo establish an HPLC method for the determination of paeoniflori in Fukang capsules. MethodsAnalytical column: Reliasil C18 (5μ m, 250 mm * 4.6 mm), protective column: (5 μ m, 12.50 mm * 4.6 mm); mobile phrase: acetonitrile - 0.02mol * L-1phosphate buffer (26:74); detective wavelength was at 230 nm; temperature was at 30 ℃ ; the flow rate was about 1.0 ml * min-1. ResultsThe standard curve showed a good linearity in the range of 0.496 ~ 29.76 μ g * ml-1, r = 0.999 5. The average recovery was 99.82% and RSD was 2.11%. ConclusionThe method is feasible and simple to operate, and it is suitable for the quality control of Fukang capsules. Keywords:: Paeoniflori; Fukang capsules; HPLC Salvia Fukang granules based on Chinese medicine theory, the use of modern technology, made preparations containing Chinese angelica, red sage root, red peony, safflower, etc. 5 flavor compound preparation of Chinese medicine herbs, which prescriptions in the treatment of irregular menstruation, dysmenorrhea, amenorrhea, postpartum abdominal pain, congestion infertility, etc. effect is significant [1]. TPG is the side