RP-HPLC determination of phase collected in different parts of the content of alizarin in madder.docVIP

 PAGE \* MERGEFORMAT 7 RP-HPLC determination of phase collected in different parts of the content of alizarin in madder [Abstract] Objective RP-HPLC (RP  HPLC) determination in different regions and different leaf collection period alizarin madder Zhongshan University content. Method column Lichrosorb C18 column (150 mm * 4.6 mm, 5 μ m); UV detection wavelength 250 nm; mobile phase was methanol -0.1% phosphoric acid solution (85:15), flow rate 0.8 ml * min-1, the column temperature was 35 ℃ . Results mollugin in the 0.021 ~ 0.105 μ g has a good linear relationship, r = 0.999 5, the average recovery was 99.82%, RSD of 0.85% (n = 5). Conclusion The method is simple and quick, high precision and accurate. [Keywords:] madder; mollugin; RP-HPLC Abstract: ObjectiveTo develop an RP-HPLC method for determination of the contents of mcilugin in radixrubiae of the different resources and different collect times. MethodsThe extracts were seperated on Lichrosorb C18 (150 mm * 4.6 mm, 5 μ m) column with methanol-0.1% phosphoric acid (85:15) as mobile phase. The flow rate was 0.8 ml * min-1. The detection wavelength was at 250 nm. Column temperture was 35 ℃ . ResultsThe method was linear within the range of 0.021 ~ 0.105 μ g (r = 0.999 5). The average recoveries of assay were 99.82% and RSD 0.85% (n = 5). ConclusionThe method is simple, rapid and accurate. Keywords:: Radixrubiae; Mcilugin; RP-HPLC Madder as the commonly used traditional Chinese medicine for the Rubiaceae Rubia Rubia cordifolia L. dry root and rhizome, with cooling blood, bleeding, expectorant, pass through and so the effectiveness of clinical practice for the treatment of vomiting blood, uterine bleeding, traumatic bleeding, amenorrhea silt resistance, joint Bitong, bone-setting swelling [1]. Rubia main produced in Weinan, Shaanxi, Henan, Song County, Anhui, Jiangsu, Shandong and other places. Madder alizarin is one of the active ingredients, in vitro experiments show that the alizarin against Stap