RP-HPLC determination of Prunella vulgaris in two kinds of triterpene acid content.docVIP

 PAGE \* MERGEFORMAT 6 RP-HPLC determination of Prunella vulgaris in two kinds of triterpene acid content [Keywords:] Prunella vulgaris;,, ursolic acid;,, oleanolic acid;,, RP-HPLC Abstract: Objective To establish the simultaneous determination of Prunella vulgaris in ursolic acid and oleanolic acid method. Methods RP-HPLC method. Column for the Nucleodur C18 column (4.6 mm * 250 mm, 5 μ m), mobile phase was methanol: water (86:14), flow rate 0.8 ml / min, detection wavelength was 210 nm, column temperature was 25 ℃ . Results Ursolic acid into the sample volume of 0.425 2 ~ 3.826 8 μ g, oleanolic acid into the sample volume of 0.127 2 ~ 1.144 8 μ g within the linear relationship was good, ursolic acid, oleanolic acid increases in the average recovery of samples rates were 98.0% and 98.7%, RSD were 1.0% and 1.4% respectively. Conclusion The method is simple and accurate, reproducible, suitable for quality control of medicinal herbs Prunella vulgaris. Keywords: Prunella vulgaris; Ursolic acid; oleanolic acid; RP-HPLC Determination of Two Triterpene Acids in Prunella vulgaris L. by RP-HPLC Abstract: ObjectiveTo develop a new method for simultaneous determination of ursolic acid and oleanolic acid in Prunella vulgaris L. MethodsThe high performance liquid chromatography was used. The column was Nucleodur C18 (4.6 mm * 250 mm, 5 μ m) column, the mobile phase consisted of methanol - water (86:14), the flow rate was 0.8 ml / min and the detection wavelength was set at 210 nm. ResultsThe linear range with good linearity of ursolic acid was between 0.425 2 ~ 3.826 8 μ g and with the oleanolic acid was between 0.127 2 ~ 1.144 8 μ g. The average recovery of ursolic acid was 98.0% with relative standard deviation (RSD) of 1.0% and the oleanolic acid was 98.7% with RSD of 1.4%. ConclusionThe method is simple, accurate, reliable and can be used for quality control of Prunella vulgaris L. Keywords:: Prunella vulgaris L; Ursolic acid; Oleanolic acid; RP-HPLC Prunella