RP-HPLC determination of ipratropium bromide inhalation solution with the content of.doc

 PAGE \* MERGEFORMAT 13 RP-HPLC determination of ipratropium bromide inhalation solution with the content of Author: Lu Lai-Chun, Zhang Rong, Liu Tong-hua, JIANG Xue-wen 【Abstract】 Objective: RP-HPLC determination of ipratropium bromide inhalation solution with the content. Methods: The column was phenomenex  C8 (4.6 mm × 250 mm, 5 μm), mobile phase of acetonitrile  0.25% sodium n-heptane solution (29:71) with phosphoric acid adjusted pH value to 3.2; detection wavelength was 210 nm; a flow rate of 1.0 mL / min; injection volume 20 μL . Results: Ipratropium bromide in 12.8 ~ 256 μg / mL and the peak area was within the framework of a good linear relationship, r = 0.9995, day, day RSD were less than 1.5%. Conclusion: This method is accurate and reliable and can be used ipratropium bromide inhalation solution with the quality control of Keywords: ipratropium bromide; determination; high performance liquid chromatography Ipratropium bromide is a kind of anti-cholinergic properties of tetravalent ammonium compounds, competitive inhibition of acetylcholine on airway smooth muscle contraction. By inhibiting the intracellular cGMP production, reducing cAMP degradation, reducing the concentration of intracellular calcium, so that the airway smooth muscle relaxation, decreased mast cell release of inflammatory mediators to end the expansion of airway, breathing resistance decreases. Inhaled ipratropium bromide, the role is limited to the bronchial lung expansion, rather than acting on the body, mainly aerosol inhalation [1]. Ipratropium bromide inhalation solution used in the clinical application of more extensive, mainly in compression or ultrasonic inhalation aerosol inhalation, commonly used in the prevention and treatment of bronchial asthma in children. This paper examines the content of ipratropium bromide HPLC determination method for the preparation of the quality control methodology based on. An apparatus and materials