RP-HPLC determination of hesperidin content Huoxiangzhengqi Tablets.docVIP

 PAGE \* MERGEFORMAT 9 RP-HPLC determination of hesperidin content Huoxiangzhengqi Tablets Author: Feng Su-Xiang, Shu-Mei Wang, Liang-wang, FU Li Jiao [Abstract] Objective To establish Huoxiangzhengqi film method for the determination of hesperidin. Methods C18 column (200 mm * 4.6 mm, 5 μ m), mobile phase was methanol - acetic acid - water (volume ratio 35:4:61), flow rate: 1.0 mL / min, detection wavelength: 283 nm. The results of hesperidin in 0.0895 ~ 0.716 μ g integral value between the peak area with a good linear relationship between the average recovery was 99.16%, RSD = 1.85% (