RP-HPLC determination of hesperidin content Huoxiangzhengqi Tablets.doc

 PAGE \* MERGEFORMAT 9 RP-HPLC determination of hesperidin content Huoxiangzhengqi Tablets Author: Feng Su-Xiang, Shu-Mei Wang, Liang-wang, FU Li Jiao [Abstract] Objective To establish Huoxiangzhengqi film method for the determination of hesperidin. Methods C18 column (200 mm * 4.6 mm, 5 μ m), mobile phase was methanol - acetic acid - water (volume ratio 35:4:61), flow rate: 1.0 mL / min, detection wavelength: 283 nm. The results of hesperidin in 0.0895 ~ 0.716 μ g integral value between the peak area with a good linear relationship between the average recovery was 99.16%, RSD = 1.85% (n = 6). Conclusion The method is simple, accurate, and can be used for Huoxiangzhengqi film quality control. [Keywords:] Huoxiangzhengqi tablets; hesperidin; high-performance liquid chromatography Abstract: Objective To establish a RP-HPLC method for determination of hesperidin in Huoxiangzhengqi tablets. Methods The sample was analyzed on a C18 column (200 mm * 4.6 mm, 5 μ m), using methanol-glacial acetic acid-water (35:4 : 61) as the mobile phase. The flow rate was 1.0 mL / min and the wavelength 283 nm. Results The average recovery was 99.16% with RSD 1.85%. Conclusion The method is simple and accurate, which can be used for the quality control of the tablets. Keywords:: Huoxiangzhengqi tablets; hesperidin; HPLC Huo Xiang Zheng Qi-chip-speaking countries the standard compilation of proprietary Chinese medicines, the Department of Pulmonary Medicine (1) volumes containing relevant species [1], from patchouli, tangerine peel, basil leaf oil, 10 Chinese herbal medicines, has a solution of table dampness, qi, and the the effect of, for exogenous cold, wet internal injuries caused by hysteresis, or the summer cold injury Shu Shi, Zheng Jian headaches faint heavy Xiongge swelling of the liver nausea, abdominal distension Zhongwan pain, vomiting and diarrhea; Gastrointestinal symptoms were the above-mentioned strain of influenza. In order to ensure drug quality, improve pharma