RP-HPLC determination of joints g Bi Pills emodin.docVIP

 PAGE \* MERGEFORMAT 13 RP-HPLC determination of joints g Bi Pills emodin Keywords: reversed-phase high performance liquid chromatography;,, joints g Bi pills;,, emodin Abstract: Objective To establish a reversed-phase high performance liquid chromatography (RP-HPLC) determination of joints g Bi Pills emodin. Methods Zobax C18 column (4.6 mm × 150 mm, 5 μm), methanol 0.1% phosphoric acid (70:30) as mobile phase, flow rate 1.0 ml / min, detection wavelength 282 nm, column temperature was 40 ℃. The results of emodin in 0.01 ~ 0.2 mg / ml range a good linear relationship (r = 0.999 9), the average recovery was 97.56%, RSD 1.51%. Conclusion The method is simple, accurate, reliable, and can be used as joints g Bi one of the criteria of quality control pills. Keywords: RP-HPLC; joint g Bi pill; emodin Determination of Emodin in Guanjiekebi Pill by RP-HPLC Abstract: ObjectiveAn RP-HPLC method was established for the determination of emodin content in Guanjiekebi pill.MethodsZobax C18 (4.6 mm × 150 mm, 5 μm) was used with a mobile phase consisted of methanol-0.1% phosphoric acid (70:30) . The detection wavelength was 282 nm, the flow rate was 1.0 ml / min, and the temperature of the column was 40 ℃. ResultsThe linear range of emodin was 0.01 ~ 0.2 mg / ml (r = 0.999 9), while the average recovery was 97.56% (RSD = 1.51%). ConclusionThe method is simple, sensitive and accurate.It can be applied in quality control of Guanjiekebi pill. Keywords:: RP-HPLC; Guanjiekebi pill; Emodin Joint Bi pills from Chuanwu g (system), Aconitum (system), Polygonum cuspidatum, single live, Gentiana, ephedrine, composed of 15 Chinese herbal medicines, with Qufeng cold-dispelling, reviving the analgesic efficacy [1]. Mainly used in the clinical arthritis, limb pain, stretching the negative. Its quality standards, a lack of determination methods. In this paper, RP-HPLC method [2] determined that certain Chinese medicine Emodin in Polygonum cuspida