RP-HPLC determination of nasosinusitis hub of ferulic acid in Nasal Drops.docVIP

 PAGE \* MERGEFORMAT 15 RP-HPLC determination of nasosinusitis hub of ferulic acid in Nasal Drops Author: Lee Chun-hua, YAN Yu-ping, SONG Xiao-yu, Fan essence 【Key Words】 Nasosinusitis-shu nasal drops;,,Ferulic acid;,,RP-HPLC Abstract: Objective To establish a reversed-phase high performance liquid chromatography nasosinusitis axial nose drops of ferulic acid method. Methods RP-HPLC column was Diamonsil C18 column (200 mm × 4.6 mm, 5 μm); methanol-acetic acid (35:65:1 V / V / V) as mobile phase; flow rate 1.0 ml / min; detection wavelength was 322 nm; external standard method. The results of ferulic acid in the range of 0.208 ~ 1.040 μg sample volume within the X (μg) and the peak area Y was a good linear relationship, Y = 1 704 575.96 X-13 177.4 (r = 0.999 7, n = 5), the average recovery rate of 97.12%, RSD = 1.28%. Conclusion RP-HPLC determination of ferulic acid in nasal drops solution for the preparation of quality control method is simple, sensitive and accurate. Keywords:: Bi-yuan-shu nasal drops; ferulic acid; RP-HPLC Determination of the Content of Ferulic Acid in Biyuanshu Nose Drops by RPHPLC Abstract: ObjectiveTo establish the RPHPLC method for content determination of ferulic acid in Biyuanshu nose drops.MethodsThe assay was performed on a diamonsil C18 column by UV detection at the wavelength of 322 nm with methanolwateracetic acid (35:65:1) as the mobile phase at the flow rate of 1.0ml/min.ResultsThe content of ferulic acid was linear with its area over the range from 0.208 μg ~ 1.04 μg, Y = 1 704 575.96X-13 177.4 (r = 0.999 7, n = 5). The average recovery was 97.12%, RSD = 1.28%. ConclusionThe present method is convenient, sensitive and accurate with good reproducibility and can be used for the quality control of Biyuanshu nose drops. Keywords:: Biyuanshu nose drops; Ferulic acid; RPHPLC Nasosinusitis shu Drops by Chuanxiong, magnolia flowers, Centipeda, Asarum etc., with Qufeng Tongqiao, heat the