RP-HPLC determination of glycyrrhizin in Mitsukoshi ephedrine content of particles.docVIP

 PAGE \* MERGEFORMAT 9 RP-HPLC determination of glycyrrhizin in Mitsukoshi ephedrine content of particles Author: Teng Liang, LI Gui-rong, SUN Dian-A, Wood Barak [Abstract] Objective To establish the Mitsukoshi Ephedra glycyrrhizic acid particles in the reversed-phase high performance liquid chromatography (RP  HPLC) assay method. Methods RP-HPLC determination of glycyrrhizin in Mitsukoshi ephedrine content of particles, Kromasil ODS  1 C18 column (5 μ m, 4.6 mm * 250 mm), mobile phase of acetonitrile  0.05M potassium dihydrogen phosphate (with three ethylamine tune pH5.75) (26.5:73.5), the detection wavelength was 250, the column temperature of 40 ℃ , a flow rate of 1.0 ml / min. Results Mitsukoshi Ephedra particles particles of Glycyrrhizin in the linear range of 3.75 ~ 154.5 μ g / ml (r = 0.999 8), plus sample recovery 100.2%, RSD = 2.5%. Conclusion RP-HPLC determination of glycyrrhizic acid with a simple, accurate, reproducible advantages for Mitsukoshi ephedra particles provide a basis for quality control. [Keywords:] high-performance liquid chromatography glycyrrhizin Mitsukoshi Ephedra particles Mitsukoshi particles from 10 to taste medicinal Ephedra (Ephedra, gypsum, bitter almonds, Banlangen, licorice, dried tangerine peel, Bupleurum, Shegan, Houttuynia, Zhejiang Fritillaria) refining. Fang licorice to make medicine. Glycyrrhizic acid is the major indicators of liquorice herbs one of the components to develop the component standards can reflect the determination and control of the intrinsic quality of the goods. This reference to the literature [1 ~ 4], established HPLC determination of glycyrrhizic acid in this product content in order to achieve an accurate quantification purposes. An instrument and reagent 1.1 Instrument Waters2690 high-performance liquid chromatography, Waters2487 UV detector, Kromasil ODS  1 C18 column (5 μ m, 4.6 mm * 250 mm) and guard column, electronic analytical balance (BS110S, Beijing Sartorius Ltd). 1.2 rea