RP-HPLC determination of fenugreek Central Plains diosgenin content.docVIP

 PAGE \* MERGEFORMAT 16 RP-HPLC determination of fenugreek Central Plains diosgenin content Author: Shu-Xian Yu, Guoqing Feng, Zhang Wei, ZHENG Xiang-juan, Hao Xiaoxia, Luo Dongmei, 【Abstract】 Objective RP-HPLC (RP-HPLC) determination of fenugreek Central Plains diosgenin content. Methods HPLC, column for the Lichrospher C18 (4.6 mm × 250 mm, 5μm), mobile phase of acetonitrile  water (8:92, V / V), detection wavelength was 203 nm. Assay results of the linear range of 0.108 ~ 1.296 μg, the recovery rate of 96.37%, RSD of 1.78% (n = 6). Conclusion The method is simple, sensitive and accurate and can be used fenugreek Central Plains diosgenin quality control. Keywords: reversed-phase high performance liquid chromatography; fenugreek; original diosgenin Abstract: Objective To establish an RP-HPLC method for the determination of the protodioscin in Trigonella foenum-graecumL .. MethodsThe analytical column was a Lichrospher C18 (4.6 mm × 250 mm, 5 μm). The mobile phase consisted of acetonitrile-water ( 8:92, v / v). The protodioscin was detected at 203 nm. ResultsThe calibration curve was linear over the range of 0.108 ~ 1.296μg (r = 0.999 9). The average recovery of the method was 96.37%, RSD was 1.78% (n = 6). ConclusionThis method is reliable and accurate, and can be used for quality control of protodioscin in Trigonella foenum-graecum L. Keywords:: RP-HPLC; Trigonellafoenum-graecumL.; Protodioscin Fenugreek Fenugreek is a legume of fenugreek Trigonellafoenum-graecumL.’s Seed, in our main produced in Guizhou, Jiangxi, Hebei, Anhui, Sichuan, Xinjiang and other places, and are cultivated throughout the north and south. Dry fenugreek seeds Wenshen, Quhan, analgesic efficacy, attending kidney virtual cold, abdominal Leng Tong, small intestine hernia, alpine athlete’s foot embolism [1]. The main chemical composition of fenugreek as steroidal saponins, flavonoids and their glycosides, triterpenoids, alkaloids, coumarins,