RP-HPLC determination of ferulic acid in red pill content.docVIP

 PAGE \* MERGEFORMAT 6 RP-HPLC determination of ferulic acid in red pill content [Abstract] Objective To establish the red pill in the determination of ferulic acid. Methods HPLC, Phenomenex C18 column, methanol: 1% glacial acetic acid (30:70) as mobile phase, column temperature at room temperature, flow rate 1.0ml/min, detection wavelength 320nm. The results of ferulic acid was within the range of 0.402 ~ 2.008μ g a good linear relationship (r = 0.9999), the average recovery (n = 5) of 99.5% (RSD = 1.2%). Conclusion The method is simple, accurate, specific, with satisfactory results. [Keywords:] RP-HPLC method of ferulic acid Red Pharmaceutical [Abstract] Objective To establish a method for the determination of ferulic acid in Hongyao tablets by RT-HPLC.Methods The determination was performed on Phenomenex C18 column with methanol-1% glacial acetic acid (30:70) as the mobile phase. The column temperature was ambient.The detection wavelength was 320nm and the flow rate was 1.0ml/min.Results There was a good linear range within 0.402 ~ 2.008μ g of ferulic acid (r = 0.9999). The average recovery rate was 99.5% with RSD 1.2% (n = 5). Conclusion The method is simple and accurate.It can be used for the quality control of Hongyao tablets. [Keywords:] RP-HPLC; Hongyao tablets; ferulic acid Red pill is 37, Chuanxiong, angelica, Angelica, Eupolyphaga insects such as traditional Chinese medicine made from Chinese herbal compound preparation, to close contained in the ‘Ministry of Health, pharmaceutical standard’ set prescription of Chinese medicine formulations 20th book, with blood circulation and pain relief, the new Quyu Health the effect. Clinical for bruises, rheumatism, numbness. Is a commonly used Chinese patent medicines. The product is only microscopic identification of existing standards, are not well reflect the quality of products, in order to ensure the product safe and effective, this article refer to the literature [1,2], for sample processing method