RP-HPLC determination of catechin content of the Central Plains Scutellaria barbata.docVIP

 PAGE \* MERGEFORMAT 14 RP-HPLC determination of catechin content of the Central Plains Scutellaria barbata 【Abstract】 Objective To establish a medicinal Scutellaria barbata protocatechuic acid method for determination of the Central Plains. Methods The sample with 30% ethanol extraction and filtration extract to reversed-phase high performance liquid chromatography (RP-HPLC) method. With DiamonsilTM C18 column (250 mm × 4.6 mm, 5 μm), with methanol -0.05% phosphoric acid as mobile phase, flow rate of 1.0 ml * min-1, detection wavelength was 258 nm. Results protocatechuic acid retention time of about 7 min, and the other peak separation is greater than 1.5. Protocatechuic acid linear range of 0.018 ~ 0.184 μg (r = 0.999 8), the minimum detection limit was 3 ng, the average recovery and RSD were 99.7% and 0.27%. Conclusion The method is simple, rapid, accurate and reliable for the quality evaluation of medicinal Scutellaria barbata effective means. Keywords: HPLC banzhilian protocatechuic acid assay RP-HPLC Determination of Protocatechuic Acid in Scutellaria barbara Abstract: ObjectiveTo establish a method to determine the content of protocatechuic acid in Scutellaria barbara. MethodsThe sample was extracted with 30% EtOH under reflux, and then the extract solution was filtered. RP-HPLC was used for the determination of protocatechuic acid in Scutellaria barbata . The chromatographic procedure was carried out using DiamonsilTM C18 as an analytic column and a mixture of 25 volume of methanol, 75 volume of 0.05% phosphoric acid as a mobile phase at a flow rate of 1.0 ml * min-1. The detection wavelength was set at 258 nm. ResultsThe peak of protocatechuic acid appeared for about 7 minutes. Resolution of protocatechuic acid was more than 1.5 and its linear range was between 0.018 and 0.184 μg (r = 0.999 8). The minimum limit of detection was 3 ng. The average recovery and RSD value of protocatechuic acid were 99.7% and 0.27%, respectively.