RP-HPLC determination of deep-sea dragon champion wine of the contents of tanshinone Ⅱ A.docVIP

 PAGE \* MERGEFORMAT 8 RP-HPLC determination of deep-sea dragon champion wine of the contents of tanshinone Ⅱ A [Abstract] Objective To establish a champion deep-sea dragon wine tanshinone Ⅱ A method for the determination. Methods RP-HPLC determination, using C18 column (4.0 * 250mm, 5μ m), with methanol - water (70:15) as mobile phase, detection wavelength 270nm. The results of tanshinone Ⅱ A linear range of 0.06 ~ 0.18mg / ml; tanshinone Ⅱ A average recovery was 99.81%, RSD = 1.08% (n = 5). CONCLUSION The samples were measured using high sensitivity, good repeatability, can effectively control the champion deep-sea dragon wine quality. [Keywords:] champion deep-sea dragon wine; tanshinone Ⅱ A; RP-HPLC; assay Scholar wine is a deep-sea dragon Hailong, Salvia, Yang Yao sub-22 Herbs such as the composition of compound Chinese medicine preparation, with kidney essence and effect. Loss for the Kidney essence, Yaoxisuanruan, fatigue weakness, forgetfulness, insomnia, impotence and premature ejaculation and other syndromes are. The author of the prescriptions were in the determination of Salvia, Salvia contains mostly tanshinone class composition, and substituting the highest content of tanshinone Ⅱ A modern pharmacological research suggests that in order to tanshinone Ⅱ A class composition, represented by Tan has obvious pharmacological activity, so determination of first consideration to tanshinone Ⅱ A, for its determination methods more reference Pharmacopoeia [1] Determination of Danshen methods and the literature [2], the eventual adoption of RP-HPLC method was established for the content of this preparation measurement method, and through the study methodology and the three batches of samples measured results show that the measurement method is simple, feasible, reproducible, precision and good recovery to meet the requirements, the entire testing process is highly feasible, can be an objective control of the goods content, will now study the sit