RP-HPLC determination of Curcuma Curcuma oil gel alcohol content germacrone.docVIP

 PAGE \* MERGEFORMAT 4 RP-HPLC determination of Curcuma Curcuma oil gel alcohol content germacrone [Abstract] Objective: To establish a RP - high performance liquid chromatography (RP-HPLC) determination of Curcuma Curcuma oil gel alcohol content of germacrone method. Methods: In the ODS-C18 column using a mobile phase of acetonitrile at -0.025 mol / L phosphoric acid (45:55) (pH adjusted to 4.5 triethylamine) solution, gradient elution: acetonitrile: 0 ~ 5 min, 70%; 5 ~ 30 min, 70% -90%; 30 ~ 50 min, 90%. The detection wavelength was 213 nm, flow rate 1.0 ml / min. Results: 0.3 ~ 7μ g range curcumol reference substance of the peak area (Y) and its concentration (X) showed a good linear relationship, r = 0.9996, in the 0.3 ~ 7μ g range germacrone the peak area of reference substance (Y) and its concentration (X) showed a good linear relationship, r = 0.9997, average recoveries were 98.37% and 99.71% (n = 9). Conclusion: This exclusive high-simultaneous determination of gel samples Curcuma germacrone alcohol content, can be used to zedoary oil gel quality control. [Keywords:] zedoary oil Curcuma oil gel high performance liquid chromatography curcumol germacrone Determination Curcuma oil (Zedoary Turmeric Oil) from Punta Zingiberaceae Curcuma (CurcumaphaeocaulisValeton), Guangxi Ezhu (Curcunta kwangsiensis SGLee et CF Lung) Wen Yu Jin (Curcuma wenyujin YH Chen et C. Ling) of the dry roots of extracted volatile oil, was pale yellow or brownish yellow, with strong volatility [1]. Wen Yu payment for Zingiberaceae Curcuma plants, the main producing area in Ruian, Wenzhou City, Zhejiang Province, Zhejiang Province, authentic traditional Chinese medicine for the artificial cultivation of a millennium of history, the famous ‘Zhejiang Bawei’ one of the [2], which from the temperature Ezhu Chinese extract seven compounds were isolated, including three kinds of curcumin and four kinds of volatile components from oil components [3]. Ryan is now optional for local product