RP-HPLC determination of clear granules poured the contents of emodin and chrysophanol.docVIP

 PAGE \* MERGEFORMAT 6 RP-HPLC determination of clear granules poured the contents of emodin and chrysophanol [Keywords:] RP Abstract: Objective To establish a clear pouring granules of emodin, rhein method for determination of phenol. Methods RP-HPLC (RPHPLC) method, detection wavelength: 254 nm. The results of emodin linear range 0.02 ~ 0.2 μ g (r = 0.999 8), chrysophanol linear range of 0.05 ~ 0.5 μ g (r = 0.999 9), the average recovery of emodin 98.68%, RSD = 1.12%; Chrysophanol 98.44% , RSD = 0.98%. Conclusion The method is simple, accurate, reproducible, can be used as a quantitative method of quality inspection. Keywords: RP-HPLC; Qinglin granules; Emodin; Qinglin powder commonly used in clinical Chinese medicine, to close contained in the ‘Ministry of Health, pharmaceutical standard’ set prescription of Chinese medicine formulations sixth book, with heat Xiehuo, Lee water Tonglin effect. Standards have not received the content of the preparation contained in the determination of the present study, HPLC method for simultaneous determination of the preparation of two anthraquinones emodin, rhein phenol content, in order to improve the quality standards of Qinglin Granules provide a basis for. An apparatus and materials SSI PC2000 high-performance liquid chromatography, SSI Model 500 UV detector, ANASTAR chromatography workstation. Emodin, chrysophanol reference substance (Chinese medicines and biological products): Qing cream powder (a pharmaceutical factory, three different batches); methanol HPLC pure, water redistilled deionized water, the rest of analytical pure reagents. 2 Methods and Results 2.1 The chromatographic conditions were column: Lichrospher C18 (4.6 mm * 200 mm, 5 μ m), mobile phase: methanol 0.1% phosphoric acid (85:15), detection wavelength 254 nm, column temperature: 30 ℃ , flow 1.0 ml / min. 2.2 Preparation of reference substance solution were obtained precision that emodin, chrysophanol appropriate reference substance, add et