RP-HPLC Determination of Compound Tablets hyperin content Houttuynia.docVIP

 PAGE \* MERGEFORMAT 9 RP-HPLC Determination of Compound Tablets hyperin content Houttuynia Author: Huang Xiuhong, Feng Ling Ping, Liao Jianwei, Kumakichi [Abstract] Objective RP-HPLC Determination of Compound Tablets Houttuynia hyperin content. Methods HPLC external standard single point method, Kromasil C-18 column, acetonitrile -0.1% phosphoric acid (eighty-two past six p.m.) as mobile phase, column temperature of 30 ℃ , a flow rate of 1.0ml/min, λ = 360nm. Results in 0.0675 ~ 1.35μ g hyperin showed good within the linear regression equation was Y = 20.345X-12.118, R2 = 0.9999, average recovery 100.5%, RSD% of 1.4% (n = 9). Conclusion The method is simple, accurate, specific, measured results and good reproducibility. To compound the film Houttuynia Houttuynia quantitative analysis provides a scientific and effective method. [Keywords:] compound Yuxingcao tablets; hyperin; HPLC [Abstract] Objective To establish the method for detemining the content of hyperosidein Fufang Yuxingcao Pion by RP-HPLC. Methods Using Kromasil C18Column, acetonitrile-0.1% phosphoricacid solution (18:82) as the mobile phase, detection wavelength as 360nm and column temperature as 30 ℃ . The flow rate was 1.0ml/min.Results The calibrationcurve was linear at a range of 0.0675 ~ 1.35μ gfor the hyperosideand linear equation was Y = 20.345X-12.118R2 = 0.9999. The averagerecovery was 100.5% and the related standard deviation (RSD) was 1.4% (n = 9). Conclusion This method was simple and accurate and proper, which can be provide for scientific quantitative analysis method of hyeroside in Fufang Yuxingcao Pion, and the reduplicate of the result was good. [Keywords:] fufang yuxingcao tablet; hyperoside; RP-HPLC Compound Yuxingcao piece for the ‘Chinese Pharmacopoeia’ containing relevant species, is also commonly used folk medicine, has the effect of heat-clearing and detoxifying for exogenous wind-heat-induced acute Houbi, acute milk moth, Zheng Jian throat irritation, sore throat ; ac