RP-HPLC determination of antispasmodic analgesic tincture content of hesperidin in.docVIP

 PAGE \* MERGEFORMAT 13 RP-HPLC determination of antispasmodic analgesic tincture content of hesperidin in 【Abstract】 Objective To establish a reversed-phase high performance liquid chromatography (RP  HPLC) Determination of spasmolytic analgesic tincture of hesperidin content approach. Methods The C18 column, methanol: 1% acetic acid solution (40:60) as mobile phase, flow rate of 1.0 ml * min-1, detection wavelength was 283 nm, column temperature of 35 ℃. The results of hesperidin in the range of 0.102 ~ 0.765 μg good linearity, r = 0.999 6; The average recovery was 97.7%, RSD of 1.38% (n = 5). Conclusion The method is simple, rapid, accurate and reliable, high sensitivity and analgesic antispasmodic tincture can be used for quality control. Keywords: antispasmodic analgesic tincture; hesperidin; RP-HPLC Abstract: ObjectiveTo establish the determination method of hesperidin in Jiejing Zhentong Tincture by RP  HPLC.MethodsThe separation was performed on C18 column, with methanol  1? Etic acid (40:60) as mobile phase. The flow rate was 1.0 ml * min -1, the detection wavelength was at 283 nm, and the column temperature was 35 ℃. ResultsThe linear range of hesperidin was in the range of 0.102 ~ 0.765 μg, r = 0.999 6.The average recovery was 97.7%, and RSD was 1.38 % (n = 5). ConclusionThis method is rapid, sensitive and accurate, and it can be used for quality control of Jiejing Zhentong Tincture. Keywords:: Jiejing Zhentong tincture; Hesperidin; RP  HPLC Spasmolytic analgesic tincture from pepper leaching solution, dried tangerine peel leaching solution, methyl salicylate, menthol, etc. 4 Herbs structure with promoting blood circulation, analgesic efficacy for the treatment of soft tissue injury caused by neck, shoulder, waist, leg pain; of frostbite has a certain effect. Drug income is contained in the national drug standards [1], but the standard is only a chemical identification and determination of the amount of etha