RP-HPLC Determination of blood and oral liquid content of isofraxidin.docVIP

 PAGE \* MERGEFORMAT 7 RP-HPLC Determination of blood and oral liquid content of isofraxidin [Keywords:] tonic oral liquid; Acanthopanax; isofraxidin; RP-HPLC method Blood and oral solution is Astragalus, Acanthopanax senticosus compound composed of a variety of traditional Chinese medicine preparations, with Qi blood, strengthening the foundation of the effectiveness of righting. Clinical for cancer chemotherapy, radiation, benzene, radiation causes such as leukopenia and anemia. Acanthopanax for Chen Fang’s drugs, I reverse high performance liquid chromatography (RP-HPLC) determination of which isofraxidin content, method is accurate, and reproducible. An instrument and reagent Hitachi -635 High Performance Liquid Chromatography, SAGE chromatography workstation; chromatography pure acetonitrile, phosphoric acid as a superior grade of pure, other reagents were of analytical grade; isofraxidin reference substance (for identification use) to buy drugs and biological products in China Institution, Lot :0837-9701, normalized high-performance liquid chromatography determination of its content was 98.37%; Qi oral determination of blood samples from Jilin Province Jingyu Cavey Limited. Two chromatographic conditions Column: HpersilODS (250 * 4.6) mm, 5μ m (USA); mobile phase: acetonitrile -0.1% phosphoric acid solution (ten ninety); flow rate: 1.0ml.min; detection wavelength: 344nm; column temperature: room temperature; theory of tower plate number in order to isofraxidin peak calculation, should not be less than 7000. 3 for the preparation of test solution Reference substance solution: Precision Weigh isofraxidin appropriate reference substance, add methanol made from 80μ g per ml of solution as a reference substance solution. Solution for the test materials: Precision drawing this product 25ml, slowly adding anhydrous ethanol 70ml, while Kabe Zhen Yao, put it aside, sub-supernatant, residue water 10ml, full Zhen Yao, so that residue dissolved dispersed,